Cross-protection study of the nine serovars of Haemophilus paragallinarum in the Kume haemagglutinin scheme

Figures & data

Table 1. Designations, serovars and origins of H. paragallinarum strains used in the present study

Strain	Serovar	Origin	Reference
221	A-1	Japan	Kume et al. (1983)
2403	A-2	Germany	Kume et al. (1983)
E-3C	A-3	Brazil	Kume et al. (1983)
HP14	A-4	Australia	Eaves et al. (1989)
2671	B-1	Germany	Kume et al. (1983)
H-18	C-1	Japan	Kume et al. (1983)
Modesto	C-2	USA	Kume et al. (1983)
SA-3	C-3	South Africa	Kume et al. (1983)
HP60	C-4	Australia	Blackall et al. (1990b)

Table 2. Protection in groups of vaccinated and challenged chickens with each serovar of H. paragallinarum

	Protection (%) against challenge with H. paragallinarum of serovar^a
Vaccine	A-1	A-2	A-3	A-4	B-1	C-1	C-2	C-3	C-4
A-1	100^A	90^A	90^A	80^A	30^B	10^B	20^B	30^B	50^B
A-2	90^A	90^A	90^A	30^B	11^B	20^B	50^B	0^B	60^B
A-3	100^A	90^A	100^A	50^B	30^B	20^B	20^B	10^B	50^B
A-4	100^A	60^B	70^A	90^A	50^B	30^B	20^B	30^B	40^B
B-1	10^B	50^B	30^B	10^B	100^A	20^B	60^B	0^B	50^B
C-1	10^B	60^B	60^B	0^B	50^B	100^A	50^B	80^B	100^A
C-2	0^B	40^B	50^B	10^B	33^B	70^A	70^A	40^B	90^B
C-3	30^B	50^B	40^B	11^B	20^B	100^A	40^B	90^A	80^B
C-4	40^B	30^B	50^B	44^B	70^A	50^B	70^A	40^B	100^A
Control	0^B	10^B	0^B	0^B	0^B	0^B	0^B	20^B	30^B
^a Values within a column followed by different uppercase superscripts differ at the 1% level of significance.

Table 3. HI antibody titres to all nine Kume serovars of H. paragallinarum in vaccinated chickens

	Mean (log10) of HI antibody titres measured with haemagglutinin of H. paragallinarum of serovar^a
Vaccine	A-1	A-2	A-3	A-4	B-1	C-1	C-2	C-3	C-4
A-1	1.25^A	1.00^A	1.05^B	0.59^C	0.00^B	0.40^B	0.00^C	0.10^E	0.00^C
A-2	0.63^B	0.97^A	0.86^C	0.06^D	0.00^B	0.73^B	0.03^C	0.06^E	0.00^C
A-3	0.48^B	0.51^B	1.32^A	0.90^B	0.00^B	0.43^B	0.00^C	0.00^E	0.00^C
A-4	0.50^B	0.53^B	1.41^A	1.25^A	0.00^B	0.23^C	0.00^C	0.24^D	0.00^C
B-1	0.00^C	0.00^C	0.03^E	0.03^D	1.13^A	0.64^B	0.00^C	0.53^C	0.00^C
C-1	0.00^C	0.00^C	0.00^E	0.00^D	0.00^B	1.00^A	0.11^C	0.16^D	0.05^C
C-2	0.00^C	0.13^C	0.28^D	0.00^D	0.00^B	1.03^A	1.30^A	0.80^B	0.79^B
C-3	0.00^C	0.03^C	0.00^E	0.00^D	0.00^B	1.32^A	0.10^C	1.25^A	0.04^C
C-4	0.00^C	0.30^BC	0.33^D	0.00^D	0.00^B	0.96^A	0.68^B	0.41^C	1.35^A
Control	0.00^C	0.00^C	0.00^E	0.00^D	0.00^B	0.00^C	0.00^C	0.00^E	0.00^C
^a Mean values within a column followed by different uppercase superscripts differ at the 1% level of significance.

Kume , K , Sawata , A , Nakai , T and Matsumoto , M . 1983 . Serological classification of Haemophilus paragallinarum with a hemagglutinin system . Journal of Clinical Microbiology , 17 : 958 – 964 .

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Eaves , LE , Rogers , DG and Blackall , PJ . 1989 . Comparison of hemagglutinin and agglutinin schemes for the serological classification of Haemophilus paragallinarum and proposal of a new hemagglutinin serovar . Journal of Clinical Microbiology , 27 : 1510 – 1513 .

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Blackall , PJ , Eaves , LE and Rogers , DG . 1990b . Proposal of a new serovar and altered nomenclature for Haemophilus paragallinarum in the Kume hemagglutinin scheme . Journal of Clinical Microbiology , 28 : 1185 – 1187 .

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