Aerosolization of Mycoplasma synoviae compared with Mycoplasma gallisepticum and Enterococcus faecalis

Figures & data

Table 1. Bacterial counts (log₁₀ cfu/ml) of the validation of bacteriology of filters

Sample	M. synoviae	M. gallisepticum	E. faecalis
Stock	8	7.6	8.7
Gelatine filter 1	9	8.1	8.9
Gelatine filter 2	9.4	7.4	8.9
Cellulose nitrate filter 1	9.1	7	8.8
Cellulose nitratefilter 2	9	7.2	8.8

Table 2. Initial loss of M. synoviae, M. gallisepticum and E. faecalis in aerosols as measured by culture

Aerosol experiment	Dose (log10 cfu)	Time 0 min^a (log10 cfu/m^3 air)	Initial loss (log10 cfu/m^3 air)
M. synoviae 1	10.5	6.4	4.1
M. synoviae 2	10.5	6.7	3.8
M. gallisepticum 1	10.4	7.3	3.1
M. gallisepticum 2	10.4	7.2	3.2
E. faecalis 1	10.5	8.6	1.9
E. faecalis 2	10.5	8.6	1.9
^a Average of both gelatine filters.

Table 3. M. synoviae, M. gallisepticum and E. faecalis log₁₀ aerosol concentration with time and half-life

Time after aerosol generation (min)	Device	Filter system	M. synoviae				M. gallisepticum				E. faecalis
			Experiment 1		Experiment 2		Experiment 1		Experiment 2		Experiment 1		Experiment 2
			Log10 cfu/m^3 air	Half-life (min)^a	Log10 cfu/m^3 air	Half-life (min)	Log10 cfu/m^3 air	Half-life (min)	Log10 cfu/m^3 air	Half-life (min)	Log10 cfu/m^3 air	Half-life (min)	Log10 cfu/m^3 air	Half-life (min)
0	MD2	Gelatine	6.5		6.8		7		6.6		8.3		8.5
0	MD8	Gelatine	6.3		6.7		7.7		7.8		9		8.8
0	MD2	Cellulose nitrate	<4		4		4.9		4		7.8		7.5
25	MD2	Gelatine	<4.7		<4.7		5.9		5.6		8.3		8.3
25	MD8	Gelatine	<4.6	<4	<4.6	<4	6	5	6.5	6	8.5	19	8.8	75
25	MD2	Cellulose nitrate	<4		<4		<4		<4		6.5		7
^a Half-life time was based on the average results obtained with both gelatine filters.

Figure 1. Average droplet size distribution measured with a laser diffraction particle size analyser of BHI aerosols generated by three different Walther Pilot I spray-heads. The average of three aerosols analysed per spray-head is illustrated.

Table 4. Particle size distribution (D) expressed as a volume diameter (μm) of BHI aerosols produced by three different spray-heads

Percentage particle volume	D(v, 0.1)	D(v, 0.5)	D(v, 0.9)
Spray-head 1	4.45	12.58	434.18
Spray-head 2	3.93	10.78	623.84
Spray-head 3	4.12	11.54	416.42
Average particle diameter	4.17	11.63	491.48
The spectrum per spray-head represents the average of three aerosol measurements.