Figures & data
Table 1. The primer pairs used in the present study.
Figure 1. Comparison of PCR detection of ALV-J DNA prepared from C/E cells inoculated with 10-fold dilutions (10−1 to 10−7 noted 1 to 7) of three reference viruses, ADOL 6803, ADOL 5701 and Hc1, on day 7 post-infection. M, molecular weight (1 kb DNA ladder [# 15615-016; Gibco BRL USA]); –, negative control; 1018 bp and 517 bp, molecular weight of the marker in base pairs; ‘high’ and ‘low’, PCR product.
Table 2. Comparison of three methods to detect the ALV-J strains ADOL 6803, ADOL 5701 and Hc1 replication after 7 days in C/E cells
Figure 2. Comparison of field isolates with primers R5-F5. For identity of the isolates, see . For definitions, see caption for .
Table 3. PCR of laboratory strains and proviral cDNA field isolates inoculated in C/E cells with the two sets of primers
Table 4. Comparison of antigenemia, and serum antibodies by direct GSA ELISA, IF and PCR in C/E cells, inoculated with sera from ML+ and ML− flocks
Figure 3. RT-PCR of flock G serum amplified with primers H5–H7 and H5–AD1. Product of H5–H7, 545 base pairs; product of H5–AD1, 295 to 326 base pairs. For definitions, see caption for .
