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Original Articles

Characterization of recent H5 subtype avian influenza viruses from US poultry

, , , , , , & show all
Pages 288-297 | Received 01 Sep 2003, Accepted 01 Feb 2004, Published online: 19 Oct 2010

Figures & data

Table 1. Avian influenza virus isolates examined in this study

Table 2. Replication of AI viruses in experimentally infected chickens

Table 3. Comparison of HA cleavage site sequences and presence or absence of carbohydrate at amino acid position (Asn) 11 in North American H5 subtype AI viruses

Figure 1. Phylogenetic tree based on nucleotide sequences of the HA1 gene from H5 isolates from the US and other representative isolates from different countries. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.

Figure 1. Phylogenetic tree based on nucleotide sequences of the HA1 gene from H5 isolates from the US and other representative isolates from different countries. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.

Figure 2. Phylogenetic tree based on nucleotide sequences of the N2 gene from H5 isolates from the US and consensus sequences from recent California H6 and LBM H7 subtype isolates. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.

Figure 2. Phylogenetic tree based on nucleotide sequences of the N2 gene from H5 isolates from the US and consensus sequences from recent California H6 and LBM H7 subtype isolates. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.

Figure 3. Amino acid sequences comparison of the stalk region of N3. Identical sequences are denoted by dots, and gaps are indicated by dashes. The amino acid numbering is based on the consensus N3 sequence. The threonine insertion in the Texas H5N3 isolate between positions 76 and 77 is indicated by underlining.

Figure 3. Amino acid sequences comparison of the stalk region of N3. Identical sequences are denoted by dots, and gaps are indicated by dashes. The amino acid numbering is based on the consensus N3 sequence. The threonine insertion in the Texas H5N3 isolate between positions 76 and 77 is indicated by underlining.

Figure 4. Phylogenetic tree based on the nucleotide sequences of the NS gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and midpoint rooting.

Figure 4. Phylogenetic tree based on the nucleotide sequences of the NS gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and midpoint rooting.

Figure 5. Phylogenetic tree based on nucleotide sequences of the M gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates. Isolates assorting into different group than those seen in the trees based on HA, NA, and NS genes are boxed.

Figure 5. Phylogenetic tree based on nucleotide sequences of the M gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates. Isolates assorting into different group than those seen in the trees based on HA, NA, and NS genes are boxed.

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