Figures & data
Table 1. Avian influenza virus isolates examined in this study
Table 2. Replication of AI viruses in experimentally infected chickens
Table 3. Comparison of HA cleavage site sequences and presence or absence of carbohydrate at amino acid position (Asn) 11 in North American H5 subtype AI viruses
Figure 1. Phylogenetic tree based on nucleotide sequences of the HA1 gene from H5 isolates from the US and other representative isolates from different countries. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.
Figure 2. Phylogenetic tree based on nucleotide sequences of the N2 gene from H5 isolates from the US and consensus sequences from recent California H6 and LBM H7 subtype isolates. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates.
Figure 3. Amino acid sequences comparison of the stalk region of N3. Identical sequences are denoted by dots, and gaps are indicated by dashes. The amino acid numbering is based on the consensus N3 sequence. The threonine insertion in the Texas H5N3 isolate between positions 76 and 77 is indicated by underlining.
Figure 4. Phylogenetic tree based on the nucleotide sequences of the NS gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and midpoint rooting.
Figure 5. Phylogenetic tree based on nucleotide sequences of the M gene from H5 isolates from the US. The tree was generated by the maximum parsimony method with PAUP4.0b10, and is the result of a heuristic search and 100 bootstrap replicates. Isolates assorting into different group than those seen in the trees based on HA, NA, and NS genes are boxed.