Effect of type 1 fimbriae of Salmonellaenterica serotype Enteritidis on bacteraemia and reproductive tract infection in laying hens

Figures & data

Table 1. Isolation of a S. Enteritidis fimD mutant and the corresponding wild-type strain from the organs of intravenously inoculated laying hens

		Spleen		Liver		Ovary		Caeca		Blood		Oviduct
		n^b	CFU/g^c	n	CFU/g	n	CFU/g	n	CFU/g	n	CFU/g	Vagina		Uterus		Isthmus		Magnum		Infundibulum
Strain	Days p.i.^a											n	CFU/g	n	CFU/g	n	CFU/g	n	CFU/g	n	CFU/g
Wild type	1	3/3	4.1±1.3	3/3	1.9±1.6	2/3	1.2±1.3	1/3	1.3±0.6	2/3	0.7±0.6	2/3	0.7±0.6	2/3	0.7±0.6	1/3	0.3±0.6	1/3	0.3±0.6	1/3	0.3±0.6
	2	3/3	5.0±0.7	3/3	3.5±0.7	3/3	2.0±1.0	1/3	0.3±0.6	2/3	1.1±1.2	3/3	1.0±0.0	3/3	1.0±0.0	3/3	1.5±0.9	1/3	0.3±0.6	3/3	1.0±0.0
	4	3/3	4.5±0.5	3/3	2.3±0.3	2/3	1.9±1.7	2/3	0.7±0.6	0/3	0.0±0.0	2/3	0.7±0.6	3/3	1.0±0.0	1/3	0.3±0.6	0/3	0.0±0.0	2/3	0.7±0.6
	8	3/3	4.7±0.4	3/3	2.4±0.5	3/3	2.3±0.4	2/3	0.7±0.6	0/3	0.0±0.0	1/3	0.3±0.6	3/3	1.0±0.0	1/3	0.3±0.6	1/3	0.3±0.6	3/3	1.0±0.0
	14	6/6	2.6±0.5^A	5/6	1.0±0.6	3/6	1.7±2.3	0/6	0.0±0.0	0/6	0.0±0.0	0/6	0.0±0.0	0/6	0.0±0.0	0/6	0.0±0.0	1/6	0.2±0.4	1/6	0.2±0.4
	21	6/6	1.3±0.7^A	4/6	2.3±1.8	5/6	3.1±2.4	0/6	0.0±0.0	0/6	0.0±0.0	0/6	0.0±0.0	2/6	0.3±0.5	5/6	0.8±0.4	5/6	0.8±0.4	0/6	0.0±0.0
FimD mutant	1	3/3	3.7±1.4	3/3	2.4±1.2	2/3	1.6±1.4	2/3	0.7±0.6	2/3	1.2±1.3	2/3	0.7±0.6	3/3	0.7±0.6	3/3	0.7±0.6	2/3	0.7±0.6	3/3	0.7±0.6
	2	3/3	5.3±0.3	3/3	3.7±0.3	3/3	3.8±2.2	3/3	1.0±0.0	3/3	2.4±1.4	3/3	2.7±2.9	3/3	2.8±3.1	3/3	1.4±0.7	3/3	2.4±2.5	3/3	3.1±2.3
	4	3/3	4.2±1.4	3/3	2.9±0.8	3/3	2.2±1.1	3/3	1.0±0.0	3/3	1.0±0.0	2/3	1.0±1.0	2/3	0.7±0.6	2/3	0.7±0.6	2/3	0.7±0.6	1/3	0.3±0.6
	8	3/3	4.6±0.3	3/3	2.5±0.6	3/3	3.7±3.1	3/3	1.0±0.0	2/3	0.7±0.6	3/3	2.2±2.1	3/3	2.5±2.5	3/3	2.6±2.7	3/3	2.9±3.2	3/3	2.9±3.3
	14	6/6	4.0±0.3^A	6/6	2.1±2.2	5/6	2.8±2.7	6/6	1.9±1.4	4/6	0.7±0.5	2/6	0.7±1.1	4/6	1.2±1.2	4/6	0.9±0.8	3/6	0.9±1.2	3/6	0.5±0.5
	21	6/6	2.2±0.8^A	6/6	1.0±0.0	5/6	3.3±3.0	4/6	0.7±0.5	1/6	0.2±0.4	2/6	0.3±0.5	4/6	0.7±0.5	2/6	0.3±0.5	4/6	0.7±0.5	1/6	0.2±0.4
^a Isolation at days post-inoculation.
^b Number of tissue samples positive after selective enrichment/total number of samples tested.
^c Mean log10 number of Salmonella detected per gram±standard deviation (samples positive after enrichment were presumed to contain 10 CFU/g).
^A Significant difference (P<0.05) between birds inoculated with the fimD mutant and birds inoculated with the wild-type strain.

Table 2. Isolation of a fimD mutant of S. Enteritidis and the corresponding wild-type strain from the lumen of the oviduct of intravenously inoculated laying hens

		Swabs
Strain	Days p.i.	Vagina	Uterus	Isthmus	Magnum	Infundibulum
Wild type	1	0/3	0/3	0/3	0/3	0/3
	2	0/3	0/3	0/3	0/3	0/3
	4	0/3	0/3	0/3	0/3	0/3
	8	0/3	0/3	0/3	0/3	0/3
	14	0/6	0/6	0/6	0/6	0/6
	21	0/6	1/6	0/6	0/6	0/6
		0/24	1/24	0/24	0/24	0/24
FimD mutant	1	0/3	0/3	0/3	0/3	0/3
	2	1/3^a	1/3^a	1/3^a	1/3^a	1/3^a
	4	0/3	0/3	0/3	0/3	0/3
	8	1/3^a	1/3^a	1/3^a	1/3^a	1/3^a
	14	2/6^a	2/6^a	2/6^a	2/6^a	0/6
	21	2/6	1/6	1/6	0/6	0/6
		6/24^A	5/24	5/25^A	4/24	2/24
^a Positive after direct plating on BGA.
^A Significant difference (P<0.05) between birds inoculated with the fimD mutant and birds inoculated with the wild-type strain.

Table 3. Isolation of a fimD mutant of S. Enteritidis and the corresponding wild-type strain from eggs of intravenously inoculated laying hens

	Wild type			FimD mutant
Weeks p.i.	Yolk	Shell	White	Yolk	Shell	White
1	11/36	3/36	1/36	3/25	0/25	0/25
2	3/24	7/24	2/24	1/9	1/9	0/9
3	0/9	2/9	0/9	1/14	1/14	1/14
Total%	20.3	17.4^A	4.3	10.4	4.2^A	2.1
^A Significant difference (P<0.05) between birds inoculated with the fimD mutant and those inoculated with the wild-type strain.