A comparison of methods of inducing lachrymation and tear collection in chickens for detection of virus-specific immuoglobulins after infection with infectious bronchitis virus

Figures & data

Table 1. Experiment design to study the levels of immunoglobulins in tears induced by salt or carbachol, and collected by pipettes or filter paper

			Eyes for lachrymal collection
Group	Designation^a	n	Right^b	Left^c
Uninfected
Salt	C salt	8	Pipette	Paper
Carbachol	C carba	8	Pipette	Paper
IBV-infected
Salt	IBV salt	10	Pipette	Paper
Carbachol	IBV carba	10	Pipette	Paper
^aUC salt, uinfected control chickens, tears induced using salt; C carba, uninfected control chickens, tears induced using carbachol; IBV salt, IBV-infected chickens, tears induced using salt; IBV carba, IBV-infected chickens, tears induced using carbachol.
^bTears collected using micropipette and tips.
^cTears collected using filter paper.

Figure 1. IBV-specific IgA in tears induced after salt application or carbachol injection, and collected using pipette tips. No significant differences between the groups.

Figure 2. IBV-specific IgG in tears induced after salt application or carbachol injection, and collected using pipette tips. No significant differences between the groups.

Figure 3. IBV-specific IgG in tears induced after salt application or carbachol injection, and collected using filter papers. No significant differences between the groups.