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Original Articles

Development of a lavage procedure to collect lung secretions from chickens for evaluating respiratory humoral immunity

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Pages 396-398 | Received 04 Jan 2005, Published online: 18 Jan 2007

Figures & data

Figure 1. Procedure for obtaining a lavage sample from chickens. 1a: Tubing is inserted into the trachea down to the syrinx. 1b: Lavage fluid is injected into the lung and then aspirated back into the syringe.

Figure 1.  Procedure for obtaining a lavage sample from chickens. 1a: Tubing is inserted into the trachea down to the syrinx. 1b: Lavage fluid is injected into the lung and then aspirated back into the syringe.

Figure 2. 2a: Plot of the percentage of positive IgG anti-SE samples in serum and IgA anti-SE samples in the crop and lung flushes from non-infected hens (filled bar) versus infected hens (open bar) in trial 1. Numbers in parentheses represent the mean titres for each group. * Significantly different from non-infected controls (P<0.05). 2b: Plot of the percentage of positive IgA anti-SE sample lung flushes from non-infected hens (filled bar) versus infected hens (open bar) in trial 2. Numbers in parentheses represent the mean titres for each group. * Significantly different from non-infected controls (P<0.05).

Figure 2.  2a: Plot of the percentage of positive IgG anti-SE samples in serum and IgA anti-SE samples in the crop and lung flushes from non-infected hens (filled bar) versus infected hens (open bar) in trial 1. Numbers in parentheses represent the mean titres for each group. * Significantly different from non-infected controls (P<0.05). 2b: Plot of the percentage of positive IgA anti-SE sample lung flushes from non-infected hens (filled bar) versus infected hens (open bar) in trial 2. Numbers in parentheses represent the mean titres for each group. * Significantly different from non-infected controls (P<0.05).

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