Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA

Figures & data

Table 1.  Primer sets used in PCR assays

Primer set	Sequence	Genome location^a (nucleotides)	Expected amplicon size (base pairs)	Reference
1	5′-cctggctataagtatcttgg-3′	2615 to 2634	593	J. Pederson (personal communication)
	5′-gtagatgtggttgttgtagc-3′	3189 to 3208
2	5′-ccaagctacaacaaccacat-3′	3185 to 3204	539	Chang et al. (2000)
	5′-tgagcgaacatgctatggaagg-3′	3702 to 3723
3	5′-gatgtgcctggtttgtctct-3′	629 to 648	1071	This paper
	5′-tcatttttccctcctcccacc-3′	1679 to 1700
4	5′-gctctacggacctgcgaccac-3′	1545 to 1566	1071	This paper
	5′-ggaaagtacaaagcccctattatt-3′	2594 to 2616
5	5′-gatgtgcctggtttgtctct-3′	629 to 648	2005	This paper
	5′-ccaacatacttatagccagg-3′	2615 to 2634
6	5′-atagctatgtctacttttttag-3′	2444 to 2465	2206	Chu et al. (2001)
	5′-aatttacagattctgagtca-3′	4632 to 4650
^aBased on the sequence published by Zadori et al. (1995) (GenBank U22967 for pairs 1 to 5 and U25749 for pair 6).

Figure 1.  Transmission electron micrograph of Muscovy duck parvovirus isolate DPV/PSU-31010 in MDEF cultures, x24,000. Bar = 100 nm.

Figure 2.  Sequence comparison for the 1109 nucleotide fragment of MDPV/PSU-31010 (GenBank accession number DQ413026) with three MDPV isolates (GenBank accession numbers U22967, X75093, and Z68272) and one goose parvovirus (Genbank accession number U25749).

Figure 3.  Phylogenetic tree based on the sequence of the 1109 nucleotide of MDPV isolate MDPV/PSU-31010 and available sequences in GenBank for MDPV and GPV. The GenBank accession numbers are shown with the parvovirus type and strain/isolate name in parentheses.

Figure 4.  Predicted amino acid sequence for VP1 (4a) and VP3 (4b) of Muscovy duck parvovirus isolates PSU-31010 and U22967. Amino acids that differ between the two isolates are denoted in bold.