In vitro antibiotic susceptibility of Dutch Mycoplasma synoviae field isolates originating from joint lesions and the respiratory tract of commercial poultry

Figures & data

Table 1.  MIC breakpoints of the tested antibiotics

Antibiotic	Susceptible (µg/ml)	Intermediate (µg/ml)	Resistant (µg/ml)
Enrofloxacin^a	≤0.5	1	≥2
Oxytetracycline^a,b	≤4	8	≥16
Tylosin^a	≤1	2	≥4
Difloxacin^c	≤0.5	1 to 2	≥4
Tilmicosin^d	≤8	16	≥32
^aBreakpoints according to Kempf et al. (1989) and Hannan (1997).
^bBreakpoints of doxycycline were expected to be comparable with that of oxytetracycline according to Kempf et al. (1989) and Hannan (1997) .
^cBreakpoints according to NCCLS standard M31-A2, based on Enterobacteriaceae and Staphylococcus spp. in dogs (skin).
^dBreakpoints according to NCCLS standard M31-A2, based on Mannheimia haemolytica in cattle (respiratory disease) and Pasteurella multocida and Actinobacillus pleuropneumoniae in swine (respiratory disease).

Table 2.  Reproducibility of the test based on the initial MIC and antibiotic susceptibility profiles at day 7 and day 14 (µg/ml) of M. synoviae WVU 1853

	Enrofloxacin			Difloxacin			Doxycycline			Tylosin			Tilmicosin
Strain	Initial MIC	Day 7	Day 14	Initial MIC	Day 7	Day 14	Initial MIC	Day 7	Day 14	Initial MIC	Day 7	Day 14	Initial MIC	Day 7	Day 14
Joint^a
WVU 1853	0.25	0.5	1	0.5	1	1	≤0.015	0.06	0.25	≤0.015	≤0.015	≤0.015	0.06	0.5	0.5
Respiratory^b
WVU 1853	0.5	0.5	1	0.25	0.5	1	≤0.015	≤0.015	≤0.015	≤0.015	≤0.015	≤0.015	≤0.015	0.125	0.25
WVU 1853	0.25	0.5	0.5	0.25	1	1	≤0.015	≤0.015	0.06	≤0.015	≤0.015	≤0.015	≤0.015	0.06	0.125
WVU 1853	0.25	0.5	1	0.125	1	1	≤0.015	≤0.015	0.03	≤0.015	≤0.015	≤0.015	≤0.015	0.125	0.125
WVU 1853	0.25	0.5	1	0.5	1	1	≤0.015	≤0.015	0.06	≤0.015	≤0.015	≤0.015	0.06	0.125	0.125
WVU 1853	0.5	0.5	0.5	0.5	1	1	≤0.015	≤0.015	0.03	≤0.015	≤0.015	≤0.015	0.06	0.125	0.125
WVU 1853	0.25	0.5	0.5	0.5	1	1	≤0.015	0.03	0.06	≤0.015	0.03	0.03	≤0.015	0.125	0.25
WVU 1853	0.125	0.5	0.5	0.25	0.5	0.5	≤0.015	0.03	0.125	≤0.015	≤0.015	≤0.015	≤0.015	0.25	0.25
WVU 1853	0.25	0.5	0.5	0.125	1	1	≤0.015	0.03	0.06	≤0.015	≤0.015	0.03	≤0.015	0.125	0.25
WVU 1853	0.25	0.25	0.5	0.5	0.5	0.5	≤0.015	0.03	0.06	≤0.015	≤0.015	≤0.015	0.06	0.25	0.25
WVU 1853	0.25	0.5	0.5	0.5	1	1	≤0.015	0.03	0.125	≤0.015	0.03	0.03	0.06	0.25	0.25
^aWVU 1853 control strain pilot MIC study.
^bWVU 1853 control strains main MIC study.

Table 3.  Initial and final MIC (µg/ml) values and corresponding antibiotic sensitivity profiles of all M. synoviae isolates tested

		Enrofloxacin		Difloxacin		Doxycyclin		Tylosin		Tilmicosin
Isolate	Source, year	Initial (profile)	Final^a	Initial (profile)	Final^a	Initial (profile)	Final	Initial (profile)	Final	Initial (profile)	Final
Joint^b
2000.05	Layer, 2000	0.25 (S)	16 (R)	0.5 (S)	4 (R)	≤0.015 (S)	0.25	≤0.015 (S)	≤0.015	0.06 (S)	0.25
2001.14	Turkey, 2001	0.25 (S)	1 (I)	0.5 (S)	1 (I)	≤0.015 (S)	0.125	≤0.015 (S)	≤0.015	0.03 (S)	0.25
2002.02	Turkey, 2002	0.25 (S)	0.25	0.25 (S)	0.5	≤0.015 (S)	0.125	≤0.015 (S)	≤0.015	0.03 (S)	0.125
Respiratory^c
2001.38	Layer, 2001	0.25/0.125^d (S)	0.25	0.25 (S)	0.25/0.5	≤0.015 (S)	0.06/0.125	≤0.015/0.06 (S)	0.125/0.25	0.03/ ≤ 0.015 (S)	0.125
2001.39	Layer, 2001	0.125 (S)	0.25	0.25 (S)	0.25	≤0.015 (S)	0.06/0.125	≤0.015/0.06 (S)	0.5/0.25	≤0.015/0.03 (S)	0.125/0.06
2001.40	Breeder, 2001	0.125/0.03 (S)	0.25/0.125	0.25/0.06 (S)	0.5	≤0.015 (S)	0.06/0.03	≤0.015 (S)	0.03/ ≤ 0.015	≤0.015/0.03 (S)	0.125/0.25
2001.41	Breeder 2001	0.5/0.125 (S)	1/0.5 (I/S)	0.5/0.06 (S)	1 (I)	≤0.015 (S)	0.125/0.03	≤0.015 (S)	≤0.015	0.06/0.03 (S)	0.25
2001.43	Layer, 2001	0.125/0.25 (S)	0.5	0.5/ ≤ 0.015 (S)	1/0.5 (I/S)	≤0.015 (S)	0.06	≤0.015 (S)	≤0.015/0.03	0.125/0.03 (S)	0.25/0.125
2002.01	Layer, 2002	0.25 (S)	0.5/1 (S/I)	0.5 (S)	1 (I)	0.03/ ≤ 0.015 (S)	0.125/0.06	≤0.015 (S)	≤0.015	0.06 (S)	0.125/0.25
2002.05	Layer, 2002	0.06/ ≤ 0.015 (S)	0.125	0.125 (S)	0.5	≤0.015 (S)	0.03/0.06	≤0.015 (S)	0.03	≤0.015/0.06 (S)	0.125/0.25
2002.11	Layer, 2002	0.25/0.125 (S)	0.5/0.25	0.5/0.25(S)	1 (I)	≤0.015 (S)	0.03/0.125	≤0.015 (S)	0.03	≤0.015/0.06 (S)	0.125
2002.15	Breeder, 2002	0.25 (S)	0.5	0.5/0.25 (S)	0.5	≤0.015 (S)	0.03	≤0.015 (S)	≤0.015	≤0.015/0.06 (S)	0.125
2002.17	Layer, 2002	0.125/0.25 (S)	0.5	≤0.015/0.5 (S)	1 (I)	≤0.015 (S)	≤0.015/0.03	≤0.015 (S)	≤0.015	≤0.015/0.06 (S)	0.25
2003.08	Breeder, 2003	0.5 (S)	8 (R)	0.25/0.5 (S)	2 (I)	≤0.015 (S)	0.06	≤0.015 (S)	0.06	0.06/0.03 (S)	0.5
2003.09	Breeder, 2003	4/2 (R)	8	0.5/2 (S/I)	4/2 (R/I)	≤0.015 (S)	0.03/0.06	≤0.015 (S)	≤0.015	0.06/ ≤ 0.015 (S)	0.125
2004.01	Breeder, 2004	0.06/0.5 (S)	0.5	0.25/0.5 (S)	0.5/1 (S/I)	0.03/ ≤ 0.015 (S)	0.125	≤0.015 (S)	0.03	0.03 (S)	0.5/0.25
2004.08	Breeder, 2004	2/4 (R)	4	0.5/1 (S/I)	2/1 (I)	≤0.015/0.03 (S)	0.06/0.03	≤0.015 (S)	≤0.015	0.125 (S)	0.5/0.25
S, susceptible; I, intermediate; R, resistant.
^aOnly MIC profiles differing from the initial are mentioned.
^bIsolates tested once.
^cIsolates tested in duplicate.
^dMIC of both tests shown only if results were different from each other.

Kempf , I. , Ollivier , C. , L'Hopitalier , R. , Guittet , M. & Bennejean . 1989 . Concentrations minimales inhibitrices de 13 antibiotiques vis-à-vis de 21 souches de mycoplasmes des volailles . Le Point Vétérinaire , 20 , 935 – 940 .

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