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ORIGINAL ARTICLES

Tissue distribution of haemolytic Gallibacterium anatis isolates in laying birds with reproductive disorders

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Pages 1-7 | Received 24 May 2008, Published online: 23 Jan 2009

Figures & data

Table 1.  Size and age of the flocks investigated

Table 2.  Main necropsy findings

Table 3.  Isolation of haemolytic G. anatis and E. coli from different organs displayed in connection with the housing system and the number of birds expressing lesions in either the ovaries and/or the oviduct

Table 4.   E. coli obtained from different organs

Figure 1. Agarose gel electrophoresis of amplification products from haemolytic G. anatis field isolates. Lane M, 100 bp DNA ladder (Invitrogen); lanes 1 and 10, isolates from cloaca; lanes 2, 6, 11 and 12, isolates from choana; lanes 3 and 8, isolates from intestine; lanes 4 and 7, isolates from trachea; lane 5, isolate from ovary; lanes 9 and 13, isolates from lungs; lanes 14 and 16, negative controls; lane 15, positive control.

Figure 1.  Agarose gel electrophoresis of amplification products from haemolytic G. anatis field isolates. Lane M, 100 bp DNA ladder (Invitrogen); lanes 1 and 10, isolates from cloaca; lanes 2, 6, 11 and 12, isolates from choana; lanes 3 and 8, isolates from intestine; lanes 4 and 7, isolates from trachea; lane 5, isolate from ovary; lanes 9 and 13, isolates from lungs; lanes 14 and 16, negative controls; lane 15, positive control.

Figure 2. Dendrogram (unweighted pair group method using arithmetic averages) of AFLP similarities of 83 Gallibacterium isolates. SD, band-based Dice similarity coefficient (%). Flocks, the flock number from which the isolates originate. Isolates grouping at a 90% similarity level or higher were pooled as indicated by grey triangles. Dotted line, clonal cut-off level at 90% similarity.

Figure 2.  Dendrogram (unweighted pair group method using arithmetic averages) of AFLP similarities of 83 Gallibacterium isolates. SD, band-based Dice similarity coefficient (%). Flocks, the flock number from which the isolates originate. Isolates grouping at a 90% similarity level or higher were pooled as indicated by grey triangles. Dotted line, clonal cut-off level at 90% similarity.

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