Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran

Figures & data

Table 1.  CAV nucleotide sequence names, Genbank accession numbers and the country of origin for the sequences used in the present study.

	Sequence name	Accession number	Country		Sequence name	Accession number	Country
1	69	DQ016140	Slovenia	10	LF4	AY839944	China
2	Lanlate.NIE/11.02/71	AJ888528	Nigeria	11	Cuxhaven-1	M55918	Germany
3	strain_BD-3	AF395114	Germany	12	01-4201	DQ991394	USA
4	09D243-South	HM018719	Korea	13	Isolate_3-1	AF390038	Malaysia
5	AH6	DQ124935	China	14	26P4	D10068	The Netherlands
6	CH-CK/05-01/GD/210	AM407817	China	15	Del-Ros	AF313470	USA
7	SH11	DQ141670	China	16	TJBD40	AY846844	China
8	Satara	EF552230	India	17	ConnB	U69548	USA
9	98D02152-Alabama	AF311892	USA

Figure 1.  Agarose gel electrophoresis of VP1 gene PCR products of CAV isolates digested with HinfI. Lane MW, molecular weight 100 bp (Fermentas, Burlington, Canada); lanes A, B, C and D, four different RFLP patterns.

Figure 2.  Molecular phylogenetic analysis of 21 nucleotide sequences of CAV VP1 gene by the maximum-likelihood method.

Table 2.  Estimates of evolutionary divergence between nucleotide sequences of the CAV VP1 gene.

		1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
1	69-Slovenia-DQ016140
2	Lanlate.NIE/11.02/71-Nigeria-AJ888528	0.005
3	strain_BD-3-Germany-AF395114	0.005	0.007
4	09D243-South_Korea-HM018719	0.010	0.010	0.013
5	AH6_China-DQ124935	0.012	0.012	0.014	0.008
6	CH-CK/05-01/GD/210-China-AM407817	0.013	0.013	0.013	0.009	0.009
7	SH11-China-DQ141670	0.014	0.014	0.017	0.013	0.017	0.016
8	Satara-India-EF552230	0.011	0.013	0.013	0.018	0.020	0.021	0.022
9	Ur-19-Iran	0.005	0.007	0.008	0.013	0.014	0.015	0.016	0.013
10	Ur-24-Iran	0.018	0.018	0.019	0.021	0.022	0.023	0.015	0.026	0.020
11	Ur-32-Iran	0.023	0.023	0.024	0.025	0.027	0.028	0.020	0.031	0.025	0.009
12	Ur-41-Iran	0.040	0.038	0.042	0.042	0.045	0.046	0.044	0.047	0.040	0.042	0.035
13	98D02152-Alabama-USA-AF311892	0.016	0.016	0.018	0.018	0.018	0.019	0.011	0.024	0.017	0.009	0.014	0.041
14	LF4-China-AY839944	0.017	0.017	0.020	0.017	0.018	0.019	0.016	0.025	0.019	0.017	0.022	0.045	0.014
15	Cuxhaven-1-M55918	0.018	0.020	0.021	0.022	0.025	0.025	0.023	0.021	0.019	0.019	0.025	0.045	0.017	0.024
16	01-4201-USA-DQ991394	0.018	0.017	0.017	0.021	0.022	0.023	0.015	0.026	0.020	0.008	0.015	0.047	0.010	0.018	0.021
17	Isolate_3-1_Malaysia-AF390038	0.019	0.021	0.021	0.024	0.025	0.026	0.023	0.024	0.020	0.019	0.025	0.047	0.017	0.024	0.005	0.021
18	26P4-The_Netherlands-D10068_D01218	0.017	0.017	0.020	0.020	0.021	0.022	0.021	0.024	0.020	0.018	0.024	0.047	0.016	0.019	0.019	0.020	0.021
19	Del-Ros-USA-AF313470	0.018	0.017	0.017	0.021	0.022	0.023	0.015	0.026	0.020	0.008	0.015	0.047	0.010	0.018	0.021	0.000	0.021	0.020
20	TJBD40-China-AY846844	0.021	0.021	0.023	0.021	0.021	0.022	0.017	0.029	0.022	0.015	0.021	0.047	0.011	0.011	0.023	0.015	0.023	0.020	0.015
21	ConnB-USA-U69548	0.021	0.022	0.023	0.025	0.027	0.028	0.025	0.025	0.021	0.021	0.027	0.047	0.019	0.026	0.004	0.023	0.009	0.021	0.023	0.025

Figure 3.  The results of bootscanning of Ur-41-Iran. The y axis gives the percentage of permutated trees using a sliding window of 200 bp wide centred on the position plotted, with a step size between plots of 20 bp. AH6, Ur-32-Iran and 26P4 strains were used as the outgroup to determine the breakpoint.