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ORIGINAL ARTICLE

Avian pox in crimson rosellas (Platycercus elegans) in southern Australia

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Pages 147-150 | Received 14 Jun 2012, Accepted 26 Nov 2012, Published online: 14 Apr 2013

Figures & data

Figure 1. Multiple, pale raised lesions on the foot of Case A.

Figure 1.  Multiple, pale raised lesions on the foot of Case A.

Figure 2. (Colour online) 2a: Proliferative skin lesion with hyperkeratosis. Biopsy from the foot of Case A. Haematoxylin and eosin stained section. 2b: Higher power version of (2a) illustrating numerous large intracytoplasmic eosinophilic inclusions within hypertrophied epidermal cells.

Figure 2.  (Colour online) 2a: Proliferative skin lesion with hyperkeratosis. Biopsy from the foot of Case A. Haematoxylin and eosin stained section. 2b: Higher power version of (2a) illustrating numerous large intracytoplasmic eosinophilic inclusions within hypertrophied epidermal cells.

Figure 3. Intracytoplasmic viral inclusion within an epidermal cell. The inclusion consists of mature virions as well as filaments. Transmission electron microscopy, uranyl acetate–lead citrate.

Figure 3.  Intracytoplasmic viral inclusion within an epidermal cell. The inclusion consists of mature virions as well as filaments. Transmission electron microscopy, uranyl acetate–lead citrate.

Figure 4. Detail of illustrating enveloped dumbbell-shaped viral particles typical for poxvirus.

Figure 4.  Detail of Figure 3 illustrating enveloped dumbbell-shaped viral particles typical for poxvirus.

Figure 5. Gel electrophoresis of a PCR for detection of avian poxvirus core protein 4b gene. M, 100 base pairs (bp) DNA ladder (Invitrogen, Mulgrave, Victoria, Australia); lane 1, avian poxvirus isolate from crimson rosella; lane 2, canary poxvirus; lane 3, sparrow poxvirus; lane 4, fowl poxvirus; lane 5, negative control.

Figure 5.  Gel electrophoresis of a PCR for detection of avian poxvirus core protein 4b gene. M, 100 base pairs (bp) DNA ladder (Invitrogen, Mulgrave, Victoria, Australia); lane 1, avian poxvirus isolate from crimson rosella; lane 2, canary poxvirus; lane 3, sparrow poxvirus; lane 4, fowl poxvirus; lane 5, negative control.

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