Molecular characterization of turkey enteric reovirus S3 gene

Figures & data

Figure 1. Phylogenetic tree of ARV S3 ORF sequences. The TRV S3 sequences generated from this study are in bold. The three major clades are illustrated in brackets while the three turkey groups are represented with a solid line.

Table 1. ARV S3 nucleotide and amino acid percent identities among different clades.

	Clade I, goose/duck (%)	Clade II, chicken (%)	Clade III, turkey (%)
Clade I, goose/duck
Nucleotide	91.4 to 99.6	57.8 to 62.2	59.5 to 63.5
Amino acid	91.3 to 99.2
Clade II, chicken
Nucleotide		83.2 to 100	69.2 to 72.6
Amino acid	56.1 to 62.5	91 to 100
Clade III, turkey
Nucleotide			88.9 to 100
Amino acid	58.1 to 64.5	74.1 to 80.5	93.5 to 100

Table 2. Changes observed at the amino acid level in the turkey reoviruses of this study in comparison with ATCC VR 818.^a

From	To	Position	PES versus non-PES (number of birds showing the change)
Q	H	53	PES (2)
Y	C	69	PES (2)
I	V	92	PES (1)
A	T	106	PES (6), non-PES (14)
I	V	110	PES (9)
D	N	112	PES (1)
P	S	125	PES (7), non-PES (14)
E	T	126	PES (1)
D	E	128	PES (8)
R	S	138	PES (9)
Q	R	146	PES (11)
L	I	157	PES (1)
D	A	163	PES (1)
E	D	167	PES (6), non-PES (14)
K	R	174	PES (1)
K	T	174	PES (6), non-PES (14)
H	N	198	PES (3)
V	I	199	PES (3)
D	E	201	PES (1)
T	I	205	PES (1)
T	A	207	Non-PES (1)
A	V	219	PES (1)
T	D	232	PES (3)
T	A	232	PES (3), non-PES (14)
A	S	233	PES (1)
A	P	239	PES (6), non-PES (14)
F	L	244	PES (15), non-PES (14)
L	I	257	Non-PES (5)
I	V	283	Non-PES (1)
G	S	285	PES (15), non-PES (14)
K	R	287	PES (1)
S	A	290	Non-PES (1)
N	D	294	PES (1)
E	D	298	PES (6), non-PES (14)
S	A	307	PES (1)

^a A turkey enteric virus (AY444912) (Sellers et al., 2004).

Figure 2. Image of the 64 ARV sequences divided into the three major clades designated on the right side of the figure. Each colour represents a specific amino acid, while amino acids in grey match the consensus amino acid. In the consensus histogram, the dark green regions (peaks) represent conserved residues while the red regions (valleys) represent divergent amino acids. The CHCC zinc-binding motif and the dsRNA binding region are located within the rectangles at positions 51 to 75 and 287 to 293, respectively.

Sellers, H.S., Linnemann, E.G., Pereira, L. & Kapczynski, D.R. (2004). Phylogenetic analysis of the sigma 2 protein gene of turkey reoviruses. Avian Diseases, 48, 651–657. 10.1637/7181-032304R

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