Detection, epidemiology and characterization of VP6 and VP7 genes of group D rotavirus in broiler chickens

Figures & data

Figure 1. PAGE migration profile of RVD (5:2:2:2) observed in three representative study samples (lanes 1 to 3).

Table 1. Detections of RVD using RT-PCR based on the age group of chickens raised on farms in the state of Pará, Brazil.

Age (days)	Number of samples collected	RT-PCR-positive	P value^a
1 to 15	11	1	0.035
16 to 30	37	23	0.0003
31 to 45	37	6	0.008
Total	85	30	<0.005

^a Specific age group compared with the remainder of the age groups.

Figure 2. Positive results for RVD recorded in (2a) low-density and high-density houses and (2b) during the rainiest and rainy seasons.

Figure 3. Dendrogram of the partial sequences of the VP6 gene obtained from the 18 samples collected in the northern Brazilian state of Pará between 2008 and 2011. The bootstrap values (2000 pseudoreplicates) are given at the respective nodes. The sequences marked in bold were obtained in this study.

Figure 4. Dendrogram of the partial sequences of the VP7 gene obtained from 18 samples collected in the northern Brazilian state of Pará between 2008 and 2011. The bootstrap values (2000 pseudoreplicates) are given at the respective nodes. The sequences detected in this study are marked in bold.

Figure 5. Divergence of amino acid sequences between study samples and the prototype RVD 05V0049 analysed in this study. Dots, similarity to 05V0049; ?, sequence not determined. The numbers above the sequences indicate the position of amino acid protein VP7.