MALDI-TOF mass spectrometry confirms difficulties in separating species of the Avibacterium genus

Merima AlispahicClinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaCorrespondence[email protected]
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Henrik ChristensenDepartment of Veterinary Disease Biology, Faculty of Life Science, University of Copenhagen, Copenhagen, DenmarkView further author information

Magne BisgaardDepartment of Veterinary Disease Biology, Faculty of Life Science, University of Copenhagen, Copenhagen, DenmarkView further author information

Michael HessClinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaView further author information

Claudia HessClinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaView further author information

Figures & data

Table 1. Avibacterium strains analysed: sequence type, identification results by phenotype and the Bruker MALDI/Biotyper database version 3 updated with our six reference strains.

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Figure 1. Score-oriented (MSP) dendrogram generated by MALDI Biotyper 3 to determine the relationship between 33 Avibacterium strains investigated. The dendrogram was generated with following settings: distance measure was set at correlation, linkage at average and score threshold value for a single organism at 700. Strains clustering with distance levels lower than 500 could be classified up to species level.

Figure 2. MALDI-TOF MS profiles obtained from the analysis of Avibacterium strains representing different clusters from . The relative intensities of the ions are shown on the y axis, and the masses (Da) of the ions are shown on the x axis. m/z, mass to charge ratio. For a single positive charge, this value corresponds to the molecular weight of the protein.

Figure 2. MALDI-TOF MS profiles obtained from the analysis of Avibacterium strains representing different clusters from Figure 1. The relative intensities of the ions are shown on the y axis, and the masses (Da) of the ions are shown on the x axis. m/z, mass to charge ratio. For a single positive charge, this value corresponds to the molecular weight of the protein.

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MALDI-TOF mass spectrometry confirms difficulties in separating species of the Avibacterium genus

Table 1. Avibacterium strains analysed: sequence type, identification results by phenotype and the Bruker MALDI/Biotyper database version 3 updated with our six reference strains.

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MALDI-TOF mass spectrometry confirms difficulties in separating species of the Avibacterium genus

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Table 1. Avibacterium strains analysed: sequence type, identification results by phenotype and the Bruker MALDI/Biotyper database version 3 updated with our six reference strains.

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