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Original Articles

Genetic diversity and antimicrobial resistance profile of Enterococcus faecalis isolated from broilers with vertebral osteomyelitis in Southeast Brazil

, , , , ORCID Icon & ORCID Icon
Pages 14-22 | Received 06 May 2017, Accepted 18 Jul 2017, Published online: 24 Aug 2017

Figures & data

Table 1. Primer sequences used for the diagnosis of E. faecalis and detection of ARGs in the isolates from broilers with VO, using PCR.

Figure 1. Geographical distribution of E. faecalis isolated from broilers with VO from Southeast Brazil, in 2012. A, B, C, D, E, and F represent the different municipalities included in this study, which are linked to their respective boxes with details of the strains isolated from the place (strain ID, number of the flock, and sequence type number). Distance between the farms was: A–F (130 km); F–B (47 km); B–E (45 km); E–C (42 km); C–D (54 km); and D–A (161 km), comprising a total area of 10,434 km2.

Figure 1. Geographical distribution of E. faecalis isolated from broilers with VO from Southeast Brazil, in 2012. A, B, C, D, E, and F represent the different municipalities included in this study, which are linked to their respective boxes with details of the strains isolated from the place (strain ID, number of the flock, and sequence type number). Distance between the farms was: A–F (130 km); F–B (47 km); B–E (45 km); E–C (42 km); C–D (54 km); and D–A (161 km), comprising a total area of 10,434 km2.

Figure 2. Population snapshot of E. faecalis STs available in the MLST database, including strains isolated from broilers with VO from Southeast Brazil, in 2012. Each ST is represented as a dot with the ST number. Clusters of linked STs correspond to clonal complexes. Black lines connect single locus variants. Primary founders are positioned centrally in the cluster. Gray arrows indicate clusters with STs available in the E. faecalis database that were also identified among the isolates described in this study. STs pointed by black arrows are newly described in this study.

Figure 2. Population snapshot of E. faecalis STs available in the MLST database, including strains isolated from broilers with VO from Southeast Brazil, in 2012. Each ST is represented as a dot with the ST number. Clusters of linked STs correspond to clonal complexes. Black lines connect single locus variants. Primary founders are positioned centrally in the cluster. Gray arrows indicate clusters with STs available in the E. faecalis database that were also identified among the isolates described in this study. STs pointed by black arrows are newly described in this study.

Table 2. ST, antimicrobial susceptibility profile and ARGs detected by PCR of E. faecalis isolated from VO in broilers from Minas Gerais, Brazil (2012).

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