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Original Articles

Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR

Heping HuangfuHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaCorrespondence[email protected]
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Wenbo XuHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Hongkui WangHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Qing DongHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Hongwei GuoHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Yanting SunHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Yunxia LiHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Wenwen GaoHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Wuqing WangHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Jia ZhangHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Jingke ShiHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Haochun PanHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Congcong LiHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

Linkang WangHenan University of Animal Husbandry and Economy, Zhengzhou, Henan, People’s Republic of ChinaView further author information

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Figures & data

Table 1. Basic information of bacterial strains used in this study.

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Table 2. Primer and probe sequences.

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Figure 1. Amplification curves for qPCR of known standards. Curves 1–5 correspond to the concentrations of 2.53 × 10⁶, 2.53 × 10⁵, 2.53 × 10⁴, 2.53 × 10³, and 2.53 × 10² copies/µl of recombinant plasmid DNA, respectively.

Figure 1. Amplification curves for qPCR of known standards. Curves 1–5 correspond to the concentrations of 2.53 × 106, 2.53 × 105, 2.53 × 104, 2.53 × 103, and 2.53 × 102 copies/µl of recombinant plasmid DNA, respectively.

Figure 2. Specificity of qPCR. Curve 1 corresponds to G. anatis and the positive control sample; curve 2 corresponds to other bacteria and the negative control.

Figure 3. Sensitivity testing. (a) Sensitivity of the newly developed qPCR method. Curves 10⁸–10⁰ correspond to 2.53 × 10⁸–2.53 × 10⁰ copies/µl of recombinant plasmid DNA, respectively; N, negative control. (b) Sensitivity of conventional PCR detection using the same primers. M, DL2000 DNA marker; 10⁹–10⁰, 2.53 × 10⁹–2.53 × 10⁰copies/µl of recombinant plasmid DNA; N, negative control.

Figure 3. Sensitivity testing. (a) Sensitivity of the newly developed qPCR method. Curves 108–100 correspond to 2.53 × 108–2.53 × 100 copies/µl of recombinant plasmid DNA, respectively; N, negative control. (b) Sensitivity of conventional PCR detection using the same primers. M, DL2000 DNA marker; 109–100, 2.53 × 109–2.53 × 100 copies/µl of recombinant plasmid DNA; N, negative control.

Table 3. Intra- and inter-group qPCR repeatability results.

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**Table 4. Detection of G. anatis in several poultry species.**

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Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR

Table 1. Basic information of bacterial strains used in this study.

Table 2. Primer and probe sequences.

Table 3. Intra- and inter-group qPCR repeatability results.

**Table 4. Detection of G. anatis in several poultry species.**

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Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR

Figures & data

Table 1. Basic information of bacterial strains used in this study.

Table 2. Primer and probe sequences.

Table 3. Intra- and inter-group qPCR repeatability results.

Table 4. Detection of G. anatis in several poultry species.

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**Table 4. Detection of G. anatis in several poultry species.**