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Avian Pathology Volume 49, 2020 - Issue 1
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Original Articles

In vitro characterization and genetic diversity of Bordetella avium field strains

Rebecca Knaba Clinic for Poultry, University of Veterinary Medicine, Hannover, GermanyView further author information
,
Henning Petersena Clinic for Poultry, University of Veterinary Medicine, Hannover, GermanyView further author information
,
Hsuen-Ju Linb Amedes Genetics, MVZ Endokrinologikum Berlin, Berlin, GermanyView further author information
,
Martin Meixnerb Amedes Genetics, MVZ Endokrinologikum Berlin, Berlin, GermanyView further author information
,
Silke Rautenschleina Clinic for Poultry, University of Veterinary Medicine, Hannover, GermanyView further author information
&
Arne Junga Clinic for Poultry, University of Veterinary Medicine, Hannover, GermanyCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-9866-6841View further author information
ORCID Icon
Pages 36-46 | Received 03 Apr 2019, Accepted 20 Aug 2019, Published online: 10 Sep 2019

Figures & data

Table 1. B. avium isolates used in this study.

Figure 1. Adherence assay in TOCs. Number of adhered bacteria following inoculation with 105 CFU (A) and 107 CFU (B) of Bordetella avium per TOC. Bars represent the median (n = 12).

Figure 1. Adherence assay in TOCs. Number of adhered bacteria following inoculation with 105 CFU (A) and 107 CFU (B) of Bordetella avium per TOC. Bars represent the median (n = 12).

Figure 2. Ciliostasis assay in TOCs. Ciliary activity following inoculation with 105 CFU (A) and 107 CFU (B) of B. avium per TOC. Symbols represent the median (n = 12).

Figure 2. Ciliostasis assay in TOCs. Ciliary activity following inoculation with 105 CFU (A) and 107 CFU (B) of B. avium per TOC. Symbols represent the median (n = 12).

Figure 3. MST based on cgMLST allelic profiles of 17 B. avium isolates. Each circle represents an allelic profile based on sequence analysis of 2667 genes. The strains with reduced virulence-associated properties are coloured in light grey, while strains with more marked virulence-associated properties are coloured in dark grey. The numbers on the connecting lines illustrate the number of target genes with differing alleles.

Figure 3. MST based on cgMLST allelic profiles of 17 B. avium isolates. Each circle represents an allelic profile based on sequence analysis of 2667 genes. The strains with reduced virulence-associated properties are coloured in light grey, while strains with more marked virulence-associated properties are coloured in dark grey. The numbers on the connecting lines illustrate the number of target genes with differing alleles.

Figure 4. MST based on multilocus sequence typing (MLST) allelic profiles of 17 B. avium isolates. Each circle represents an allelic profile based on sequence analysis of 119 virulence-related genes. The strains with reduced virulence-associated properties are coloured in light grey, while strains with more marked virulence-associated properties are coloured in dark grey. The numbers on the connecting lines illustrate the number of target genes with differing alleles. Target genes differing between the strains with reduced virulence-associated properties and their next neighbour are listed in the table within the figure.

Figure 4. MST based on multilocus sequence typing (MLST) allelic profiles of 17 B. avium isolates. Each circle represents an allelic profile based on sequence analysis of 119 virulence-related genes. The strains with reduced virulence-associated properties are coloured in light grey, while strains with more marked virulence-associated properties are coloured in dark grey. The numbers on the connecting lines illustrate the number of target genes with differing alleles. Target genes differing between the strains with reduced virulence-associated properties and their next neighbour are listed in the table within the figure.

Figure 5. Neighbour-joining tree based on amino acid sequence alignment of putative filamentous haemagglutinin protein (BAV_RS09685) of the 17 B. avium isolates. Strains with reduced virulence-associated properties are marked by black boxes.

Figure 5. Neighbour-joining tree based on amino acid sequence alignment of putative filamentous haemagglutinin protein (BAV_RS09685) of the 17 B. avium isolates. Strains with reduced virulence-associated properties are marked by black boxes.
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