Abstract
A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC50 and IC10 of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos.
Acknowledgments
This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (NO2009BADB9B09) and the National “863” High-tech R&D Project of China “Application of molecular imprinting technique on the determination of pesticide residues” (No. 2008AA10Z422).