Antibiotic resistance and virulence factors of Escherichia coli from eagles and goshawks

Abstract

One of the major global problems in medicine is microbial resistance to antibiotics (antimicrobial resistance) and this has become an increasingly frequent research topic. This study focuses on antimicrobial resistance, phylogenetic and genetic characterization of Escherichia coli from wild birds: ten isolates from eagles (Aquila chrysaetos), nine from goshawks (Accipiter gentilis) and 24 from broilers in the Slovak Republic. Twenty-two strains with presence of int1 gene were selected and examined for the presence or absence of transposon gene (tn3), genes of antibiotic resistance and virulence factors. We detected sequence type (ST) in eagles ST 442 with genes iss, papC, iutA, cvaC, tsh, fyuA, iroN, kps, feoB, sitA, irp2, ireA for virulence factors and tetA, sul1, sul2, dfrA, aadA for antibiotic resistance; in goshawks ST 1011 with iss, papC, fyuA, iroN, feoB, sitA and qnrS1, tetA, sul1, sul2, dfrA, aadA, respectively. These ST types have been found in humans too and should be evaluated further for possible zoonotic potential and transfer of resistance genes from the environment.

Keywords:

• Escherichia coli
• APEC
• phylogenetic groups
• goshawks
• eagles
• broilers
• virulence genes
• antibiotics resistance genes
• antibiotics resistance
• biofilm formation

Acknowledgment

The authors would like to thank Dr. Karpiskova from Veterinary research institute, Brno for MLST.

Additional information

Funding

This work was supported by Slovak grant VEGA 2/0085/18 and APVV – 16 – 0171.