Assessment of phytotoxicity and impact on the enzymatic activity of soil microorganisms caused by veterinary antibiotics used in Brazilian farms

Abstract

This work aimed to evaluate the impact of veterinary antibiotics on biomass phytoproductivity and soil enzyme activity. The soil was sampled in the city of Camboriú (state of SC, Brazil). The soil enzyme activity was assessed through hydrolysis of fluorescein diacetate (FDA), while phytotoxicity was tested using Lactuca sativa (lettuce). Results showed that the most appropriate exposure time to assess the impact of antibiotics on soil microbiology was 24 h, while the incubation time of 3 h was the most appropriate for FDA hydrolysis. Ampicillin and Amoxicillin at the tested concentrations did not interfere with the enzyme activity of the soil microbiota, while Oxytetracycline and Neomycin showed a significant reduction in soil enzyme activity. For the dry and wet biomass of lettuce, 2% Colistin and 1% Ampicillin were the treatments that reduced lettuce biomass. Hence, the use of excessive antibiotics in animal production may lead to environmental impacts and, in the future, to public health problems.

Keywords:

• Antibiotics
• animal waste
• phytotoxicity
• fluorescein
• soil microbiology

Acknowledgments

CA Somensi gratefully acknowledges the grant from SETEC/MEC-Brasil (Public Call Notice 02/2020 - IFES) and Instituto Federal Catarinense (IFC Notice 39/2020). CM Radetski gratefully acknowledges the grant from CNPq-Brasil (302124/2019-5).