Calcium alginate nanoparticle crosslinked phosphorylated polyallylamine to the controlled release of clindamycin for osteomyelitis treatment

Abstract

Osteomyelitis is one of the infections of the bone, and the treatment needs to the infection problems. Here, a local therapeutic approach for efficient drug delivery systems was designed to enhance the antibiotic drug’s therapeutic activity. Calcium-Alginate nanoparticle (Ca-Alg) crosslinked phosphorylated polyallylamine (PPAA) was prepared through the salting-out technique, and it achieved 82.55% encapsulation of Clindamycin drug. The physicochemical characterizations of FTIR, SEM/EDX, TEM, and XRD were investigated to confirm the materials nature and formation. Clindamycin loaded Ca-Alg/PPAA system showed sustained Clindamycin release from the carrier. Cell viability was assessed in bone-related cells by Trypan blue assay and MTT assay analysis method. Both assay results exhibited better cell viability of synthesized materials against MG63 cells. MIC value of Ca-Alg/PPAA/Clindamycin in the Methicillin-resistant Staphylococcus aureus (MRSA) pathogen was 275 µg/mL, and it was 120 µg/mL for Enterobacter cloacae pathogen. The materials promising material for Osteomyelitis affected bone regeneration without any destructive effect and speedy recovery of infected parts from these investigations.

Keywords:

• Calcium alginate
• C. elegans
• clindamycin
• phosphorylated polyallylamine
• osteoblast like cell

Acknowledgments

M. Rajan acknowledges the PURSE program for the purchase of SEM and FT-IR and UPE programs for the purchase of TEM.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

M. Rajan is acknowledged of the financial support under the research projects from ‘ICMR’ [F.NO.5/3/8/350/2018-ITR; New Delhi, India] and Science and Engineering Research Board [Ref: YSS/2015/001532; New Delhi, India].