Maintenance of root water uptake contributes to salt-tolerance of a wild tomato species under salt stress

ABSTRACT

Under salt stress, the regulatory mechanism of root water uptake in tomato species with different salt-tolerance remains unclear. Here, we compared water uptake, aquaporin expression and oxidative damage in roots of wild tomato Solanum pimpinellifolium ‘L03708ʹ and cultivated tomato Solanum lycopersicum ‘M82ʹ under salt stress (150 mM NaCl) and H₂O₂-caused oxidative stress which is usually induced by salt stress. The results showed that the seedling growth was inhibited and root hydraulic conductance in ‘M82ʹ was decreased under salt stress, whereas these were unchanged in ‘L03708ʹ. Under salt stress, the PIP1 aquaporin abundance was decreased in ‘M82ʹ but unchanged in ‘L03708ʹ, and the PIP2 abundance was decreased in both species. The membrane damage and hydrogen peroxide accumulation were more severe in ‘M82ʹ than ‘L03708ʹ under salt stress. Exogenous H₂O₂ increased the membrane electrolyte leakage and decreased the root hydraulic conductance, with the changes being more profound in ‘M82ʹ than ‘L03708ʹ. The PIP1 abundance was increased in ‘L03708ʹ but decreased in ‘M82ʹ by H₂O₂ treatment. These results suggest that the maintenance of PIP1 abundance and antioxidant defense capability in the wild tomato species may contribute to its maintenance of root water uptake and thus salt-tolerance under salt stress.

KEYWORDS:

• Tomato
• salt stress
• root hydraulic conductance
• aquaporin
• oxidative damage

Acknowledgements

We thank Prof. Yan Liang in the College of Horticulture, Northwest A&F University for providing the tomato seeds.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplemental material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (31772290, 31501751, 31501750).