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Abstract
In recent times, fluorescence-based analytical devices are getting more equipped with LEDs than conventional light sources because of their availability in a broad range of wavelengths and appropriate power outputs. Further, LEDs are compact and affordable compared to commonly used light sources. LEDs have played a key role in miniaturizing the fluorescence detection devices and bringing the instrument to the experiment, rather than vice versa. The current review summarizes the key factors in developing a sensitive, affordable, and portable LED-Induced Fluorescence (LED-IF) detection-based analytical devices and their applications in various fields. The first part of the review discuss the fundamental aspects of fluorescence as well as LEDs in general. Whereas, the second part highlights the technical considerations and the applications of sensitive LED-IF detection-based analytical devices.
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Acknowledgments
The authors thank SERB, DST, Govt. of India, New Delhi for funding (Sanction no. EMR/2017/007700). Authors would also like to thank Manipal Academy of Higher Education (MAHE), Manipal, India, and TIFAC-CORE, DST, Govt. of India for creating the necessary infrastructure and facilities at Manipal School of Life Sciences (MSLS), Manipal used in the present study. The authors would also like to thank Dr. K. Satyamoorthy, Director, MSLS, Manipal for his encouragement and support, and Dr. Nirmal Mazumder, Assistant Professor; Subhash C, Asistant Engineer; Raghushaker C R, Jackson Rodrigues, Sushmitha Srinivasan, Research Scholars, Department of Biophysics, MSLS for helping in the study.