Physiological specialization of Puccinia triticina in Canada in 2007

Figures & data

Table 1.  Frequency and distribution of virulence phenotypes of Puccinia triticina identified by infection types to selected resistance genes in Canada in 2007

Virulencephenotype	Avirulence/virulenceformula (Lr genes)^a	BC+AB^b		SK+MB		ON		QC		PEI		Canada
		#	%	#	%	#	%	#	%	#	%	#	%
MBBJ	2a,2c,9,16,24,26,3ka,11,17,30,B,18 / 1,3,10,14a,Cen	2	18.2	0	0	0	0	0	0	0	0	2	0.5
MBDS	2a,2c,9,16,24,26,3ka,11,30,18 / 1,3,17,B,10,14a,Cen	4	36.4	0	0	1	3	0	0	0	0	5	1.3
MBGJ	2a,2c,9,16,24,26,3ka,17,30,B,18 / 1,3,11,10,14a,Cen	2	18.2	0	0	0	0	0	0	0	0	2	0.5
MBPS	2a,2c,9,16,24,26,11,18 / 1,3,3ka,17,30,B,10,14a,Cen	0	0	1	0.3	0	0	0	0	0	0	1	0.3
MCNS	2a,2c,9,16,24,11,30,18 / 1,3,26,3ka,17,B,10,14a,Cen	0	0	0	0	0	0	0	0	1	25	1	0.3
MDDS	2a,2c,9,16,26,3ka,11,30,18 / 1,3,24,17,B,10,14a,Cen	0	0	3	0.9	1	3	0	0	0	0	4	1.0
MDNS	2a,2c,9,16,26,11,30,18 / 1,3,24,3ka,17,B,10,14a,Cen	0	0	2	0.6	0	0	0	0	0	0	2	0.5
MDPN	2a,2c,9,16,26,11,10,18 / 1,3,24,3ka,17,30,B,14a,Cen	0	0	1	0.3	0	0	0	0	0	0	1	0.3
MDPS	2a,2c,9,16,26,11,18 / 1,3,24,3ka,17,30,B,10,14a,Cen	0	0	17	5.3	3	9.1	0	0	0	0	20	5.2
MFDS	2a,2c,9,16,3ka,11,30,18 / 1,3,24,26,17,B,10,14a,Cen	0	0	0	0	0	0	2	20	1	25	3	0.8
MFGJ	2a,2c,9,16,3ka,17,30,B,18 / 1,3,24,26,11,10,14a,Cen	0	0	0	0	0	0	1	10	0	0	1	0.3
MFPS	2a,2c,9,16,11,18 / 1,3,24,26,3ka,17,30,B,10,14a,Cen	0	0	14	4.3	1	3	0	0	0	0	15	3.9
MFRJ	2a,2c,9,16,17,B,18 / 1,3,24,26,3ka,11,30,10,14a,Cen	0	0	0	0	2	6.1	0	0	0	0	2	0.5
MGBJ	2a,2c,9,24,26,3ka,11,17,30,B,18 / 1,3,16,10,14a,Cen	0	0	1	0.3	0	0	0	0	0	0	1	0.3
MHNQ	2a,2c,9,24,11,30,14a,18 / 1,3,16,26,3ka,17,B,10,Cen	0	0	0	0	0	0	0	0	1	25	1	0.3
MHNS	2a,2c,9,24,11,30,18 / 1,3,16,26,3ka,17,B,10,14a,Cen	0	0	0	0	0	0	0	0	1	25	1	0.3
MLDS	2a,2c,16,24,26,3ka,11,30,18 / 1,3,9,17,B,10,14a,Cen	2	18.2	17	5.3	12	36.4	1	10	0	0	32	8.4
MMDS	2a,2c,16,24,3ka,11,30,18 / 1,3,9,26,17,B,10,14a,Cen	0	0	1	0.3	0	0	0	0	0	0	1	0.3
TBNS	9,16,24,26,11,30,18 / 1,2a,2c,3,3ka,17,B,10,14a,Cen	0	0	1	0.3	0	0	0	0	0	0	1	0.3
TDBG	9,16,26,3ka,11,17,30,B,14a,18,Cen / 1,2a,2c,3,24,10	0	0	197	61	7	21.2	3	30	0	0	207	54.3
TDBJ	9,16,26,3ka,11,17,30,B,18 / 1,2a,2c,3,24,10,14a,Cen	1	9.1	49	15.2	5	15.2	3	30	0	0	58	15.2
TDBJ	9,16,26,3ka,11,17,30,B,18,Cen / 1,2a,2c,3,24,10,14a	0	0	5	1.5	0	0	0	0	0	0	5	1.3
TFBG	9,16,3ka,11,17,30,B,14a,18,Cen / 1,2a,2c,3,24,26,10	0	0	14	4.3	1	3	0	0	0	0	15	3.9
Total		11		323		33		10		4		381
Notes: ^aVirulence phenotype determined according to Long & Kolmer (1989).
^bBC = British Columbia, AB = Alberta, MB = Manitoba, SK = Saskatchewan, ON = Ontario, QC = Quebec and PEI = Prince Edward Island.

Table 2.  Frequencies of virulence of Puccinia triticin a in Canada in 2007 to lines of wheat with single Lr genes for leaf rust resistance

Resistancegene	BC+AB^a		SK+MB		ON		QC		PEI		Canada
	#	%	#	%	#	%	#	%	#	%	#	%
Lr1	11	100	323	100	33	100	10	100	4	100	381	100.0
Lr2a	1	9.1	266	82.4	13	39.4	6	60	0	0	286	75.1
Lr2c	1	9.1	266	82.4	13	39.4	6	60	0	0	286	75.1
Lr3	11	100	323	100	33	100	10	100	4	100	381	100.0
Lr9	2	18.2	18	5.6	12	36.4	1	10	0	0	33	8.7
Lr16	0	0	1	0.3	0	0	0	0	2	50	3	0.8
Lr24	1	9.1	302	93.5	20	60.6	9	90	1	25	333	87.4
Lr26	0	0	29	9	4	12.1	3	30	4	100	40	10.5
Lr3ka	0	0	36	11.1	6	18.2	0	0	3	75	45	11.8
Lr11	2	18.2	0	0	2	6.1	1	10	0	0	5	1.3
Lr17	6	54.5	57	17.6	18	54.5	3	30	4	100	88	23.1
Lr30	0	0	33	10.2	6	18.2	0	0	0	0	39	10.2
LrB	6	54.5	57	17.6	18	54.5	3	30	4	100	88	23.1
Lr10	11	100	322	99.7	33	100	10	100	4	100	380	99.7
Lr14a	11	100	112	34.7	25	75.8	7	70	3	75	158	41.5
Lr18	0	0	0	0	0	0	0	0	0	0	0	0.0
Total	11		323		33		10		4		381
Note: ^aBC = British Columbia, AB = Alberta, MB = Manitoba, SK = Saskatchewan, ON = Ontario, QC = Quebec and PEI = Prince Edward Island.

Table 3.  Frequency (%) of predominant P. triticina virulence phenotypes in Canada from 2000 to 2007

Virulencephenotype^a	Year
	2000	2001	2002	2003	2004	2005	2006	2007
KBBJ	0^c	0	0.4	9.6	0	0	0	0
MBDS	56.0	19.2	32.9	11.0	10.0	3.5	1.4	1.3
MFDS	0	0	0	0	0	0.2	5.1	0.8
MLDS	0	0	0.7	0	0	0	1.4	8.4
TBBG^b	0	0	0	0	17.4	10.9	1.7	0
TBBJ	0.6	2.1	11.5	61.6	48.0	6.1	3.4	0
TDBG^b	0	0	0	0	0.4	46.7	50.1	54.3
TDBJ	0.3	0	0	0	0.7	9.1	16.7	16.5
TGBJ	22.4	50.0	27.4	4.1	3.9	0.8	0	0
THBJ	5.6	18.5	1.7	0	0	0	0	0
Others	15.1	10.2	26.1	13.7	19.6	22.9	21.6	18.7
Data from McCallum & Seto-Goh (2003, 2004, 2005, 2006a, 2006b, 2008, 2009).
Notes: ^aVirulence phenotype determined according to Long & Kolmer (1989). Collections from Manitoba and Saskatchewan make up the majority of the collections in each of the years.
^bThese virulence phenotypes were avirulent to LrCen. All other virulence phenotypes were virulent to LrCen, except TDBJ in which a low proportion of isolates were avirulent.

Fig. 1. Frequency of virulence (%) from 2000–2007 in the Manitoba and Saskatchewan population of P. triticina to near-isogenic lines containing Lr2a, Lr14a, Lr16, Lr17 or Lr24. Data from McCallum & Seto-Goh (2003, 2004, 2005, 2006a, 2006b, 2008, 2009).

Table 4.  Infection types of 39 representative isolates from Canada in 2007 tested on near isogenic lines of ‘Thatcher’ with adult plant resistance genes and additional seedling resistance genes

Isolate number		Virulence phenotype^b	Adult plant resistance genes			Seedling resistance genes
	Region^a		Lr12	Lr13	Lr37	Lr3bg	Lr20	Lr23	Lr28	LrCen
214-2	BC	MBBJ	3+ ^c	3+	;	3+	3+	3+	3+	3+
215-1	BC	MBBJ	33−	1−	;	4	4	4	4	4
218-2	BC	MBDS	3+	3+	3+	4	4	4	;	4
215-2	BC	MBGJ	2−	3+	;	2−	4	4	4	4
189-1	MB+SK	MBPS	3+	3+	3+	4	4	4	;	4
251-1	PEI	MCNS	3+	3+	3+	4	4	2−	;	4
187-1	MB+SK	MDDS	3+	3+	3+	4	4	1−2−	;	4
189-2	MB+SK	MDNS	3+	3+	3+	4	4	1−2−	;	4
168-2	MB+SK	MDPN	2+	2−	33−	3+	4	2−	;	4
188-2	MB+SK	MDPS	3+	3+	3−	4	4	2	;	4
221-1	ON	MDPS	22−	3+	3+	4	4	22−	;1=	4
249-2	QC	MFDS	3+	3+	3+	4	4	1−2−	;1=	4
250-2	QC	MFGJ	3+	3+	;	1−2+	4	2−	4	4
181-1	MB+SK	MFPS	3+	3+	3+	4	4	2−	;	4
237-2	ON	MFPS	3+	3+	3−	4	4	22−	;	4
245-1	ON	MFRJ	3+	3	;	4	4	4	4	4
245-2	ON	MFRJ	2+	33+	;	4	4	2+	4	4
1-2	MB+SK	MGBJ	11−	1+2+	;1−	3+	4	2+3−	4	4
252-2	PEI	MHNQ	3+	3+	33+	4	4	1−	;1=	4
252-1	PEI	MHNS	3+	3+	3+	4	4	1−2−	;	4
3-1	MB+SK	MLDS	3+	3+	3+	4	4	3+4	;	4
223-1	ON	MLDS	3+	3+	3+	4	4	4	;	4
243-2	ON	MLDS	3+	3+	3+	4	4	4	;	4
247-2	QC	MLDS	3+	3+	3+	4	4	4	;	4
68-1	MB+SK	MMDS	3+	3+	3+	4	3+	4	;	4
188-1	MB+SK	TBNS	11−	3+	3+	4	4	2−	;1=	4
9-1	MB+SK	TDBG	3+	3+	3+	3+	X	4	4	X
30-1	MB+SK	TDBG	3+	3+	33−	2−	;1−X	2+	4	X
153-2	MB+SK	TDBG	3+	3+	3+	3−	;1=	3	3+	X
238-2	ON	TDBG	3+	2+3−	3+	2−	;	3+	4	X
246-1	QC	TDBG	3+	33+	3+	3+4	;+X−	4	4	X
247-1	QC	TDBG	3+	3+	3+	4	;1=	2+	4	X
18-2	MB+SK	TDBJ	3+	3+	3+	2+3−	4	2+	4	3+4
202-1	MB+SK	TDBJ	3+	3+	3−	4	4	4	4	4
244-1	ON	TDBJ	3+	3	3−	4	4	4	4	4
250-1	QC	TDBJ	3+	3+	33+	4	4	2+	4	4
100-2	MB+SK	TDBJ	3+	3+	3+	3	X	33−	3+4	X
13-2	MB+SK	TFBG	3+	3+	3+	4	X	4	4	X
198-2	MB+SK	TJBJ	3+	3+	3−	4	X−	4	4	X
Notes: ^aQC = Quebec, ON = Ontario, MB+SK = Manitoba and Saskatchewan and PEI = Prince Edward Island.
^bVirulence phenotype determined according to Long & Kolmer (1989).
^cInfection phenotypes: ‘;’ small hypersensitive flecks, ‘1’ small uredinia surrounded by necrosis, ‘2’ moderate size uredinia surrounded by chlorosis, ‘3’ medium sized uredinia without chlorosis or necrosis, ‘4’ large uredinia without chlorosis, ‘+’ larger uredinia than typical for the infection type, ‘−’ smaller uredinia than typical for the infection type, ‘=’ much smaller uredinia than typical for the infection type. ‘X’ mesothetic reaction with a range of resistant pustule types.

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