Seedling resistance to Rhizoctonia and Pythium spp. in wheat chromosome group 4 addition lines from Thinopyrum spp.

Figures & data

Table 1.  Disease severity ratings ^a for ‘Chinese Spring’ (CS), the CS-Thinopyrum elongatum amphidiploid (AgCS), chromosome 4 addition lines (CS4E, CS4J), and the 4E donor Thinopyrum elongatum (Te) after challenge with Rhizoctonia solani AG8 or R. oryzae

Genotype	R. solani AG8	R. oryzae
CS	3.7 ± 0.1	3.1 ± 0.2
AgCS	3.5 ± 0.2	3.0 ± 0.2
CS4E	2.6 ± 0.2 *	2.0 ± 0.2 *
CS4J	2.4 ± 0.1 *	2.5 ± 0.2 *
Te	1.0 ± 0.1 *	0.9 ± 0.1 *
Notes: ^a Mean and standard errors using a rating scale of 0 (no symptoms) to 8 (lesions and severe stunting in all seminal roots) after 14 days of growth in infested soil. Asterisks indicate significant (P < 0.05) differences relative to CS, determined using Kruskal–Wallis one-way ANOVA (Statistix 8.1). Data were combined from three experiments for R. solani AG8 and two experiments for R. oryzae.

Table 2.  Comparisons of root weight and root length of genotypes ‘Chinese Spring’ (CS), the CS-Thinopyrum elongatum amphidiploid (AgCS), chromosome 4 addition lines (CS4E, CS4J), and the 4E donor Thinopyrum elongatum (Te) after challenge with Rhizoctonia solani AG8 or R. oryzae

	R. solani AG8						R. oryzae^b
	Expt. 1			Expt. 2
Genotype	Control	Inoc.	Ratio^c	Control	Inoc.	Ratio^c	Control	Inoc.	Ratio^c
Root weight (mg)^a
CS	154 ± 16	111 ± 16*	0.72	94.8 ± 6	66.2 ± 4*	0.70	198 ± 11	131 ± 8*	0.66
AgCS	125 ± 11	114 ± 6	0.91	101 ± 6	78.1 ± 5*	0.77	126 ± 9	89 ± 6*	0.70
CS4E	189 ± 19	179 ± 10	0.95	86.6 ± 8	76.7 ± 6	0.89	218 ± 10	207 ± 8	0.95
CS4J	150 ± 17	190 ± 9	1.27	96.3 ± 6	79.8 ± 11	0.83	197 ± 11	179 ± 9	0.91
Te	22.5 ± 2	20.6 ± 2	0.92	8.6 ± 1.8	7.8 ± 0.8	0.91	31 ± 5	21 ± 2*	0.67
Root length (cm)^a
CS	280 ± 25	127 ± 16*	0.45	160 ± 13	102 ± 7*	0.64	250 ± 16	140 ± 8*	0.56
AgCS	208 ± 22	72.3 ± 19*	0.35	119 ± 10	88 ± 8*	0.74	116 ± 12	87 ± 7*	0.75
CS4E	269 ± 31	159 ± 12*	0.59	126 ± 11	109 ± 12	0.87	236 ± 19	203 ± 14	0.86
CS4J	215 ± 31	205 ± 15	0.95	126 ± 14	93 ± 16	0.74	197 ± 10	153 ± 11*	0.78
Te	43.2 ± 7	27.6 ± 4*	0.64	17.8 ± 3	13.6 ± 1	0.77	20 ± 3	17 ± 3*	0.86
Notes: ^a Means and standard errors after 14 days of growth in non-infested soil (Control) or soil infested with R. solani AG8 (Inoc.) or R. oryzae (Inoc.). Asterisks indicate significant (P < 0.05) decrease in inoculated compared to control within each genotype, determined using Fisher's protected least significant difference test (Statistix 8.1).
^b Data from two experiments of each pathogen were combined.
^c Ratio of inoculated to control within each genotype.

Table 3.  Reduction in Pythium ultimum disease symptoms in ‘Chinese Spring’ (CS), the CS-Thinopyrum elongatum amphidiploid (AgCS), chromosome 4 addition lines of CS (CS4E, CS4J), and the 4E donor Thinopyrum elongatum (Te)

			Ratio^a
			Total root length
Genotype	1^st leaf length^b	Root weight^b	Expt 1	Expt 2
CS	0.77	0.64	0.32	0.56
AgCS	1.07	1.09	0.74	0.71
CS4E	1.03	0.93	0.69	0.71
CS4J	0.83	0.68	0.31	0.32
Te	0.87	0.97	0.71	0.70
Notes: ^a Ratio of inoculated (800 ppg) to non-inoculated within each genotype. Data were collected after 14 days of growth in infested soil.
^b Data from two experiments were combined.