First report of Fusarium redolens from Saskatchewan and its comparative pathogenicity

Figures & data

Table 1.   Crops, sampling dates, crop growth stage and relative abundance of F. redolens as a percentage of all culturable fungi obtained in all plants from the 14 sampling sites considered in this study (samples are taken from Swift Current, SK)

Site no.	Plant (cultivar)	Previous crop	Sampling date	Plant growth stage	Per cent recovery of F. redolens %
	Cereals
1	Durum (AC Avonlea)	Pea	Aug 09	Ripening	31.5
2	Durum (AC Avonlea)	Chickpea	Aug 09	Ripening	18.8
3	Durum (AC Avonlea)	Pea	Jun 10	Booting	10.5
4	Durum (AC Avonlea)	Chickpea	Jun 10	Booting	8.8
5	Durum (AC Avonlea)	Pea	Aug10	Dough	14.2
6	Durum (AC Avonlea)	Chickpea	Aug 10	Dough	4.0
7	Durum (Eurostar)	Fallow	Aug 10	Dough	1.8
8	Durum (AC Avonlea)	Pea	Aug 10	Ripening	9.9
9	Durum (AC Avonlea)	Chickpea	Aug 10	Ripening	12.1
	Pulses
10	Pea (CDC Handle)	Spring Wheat	Jul 10	Mid-flower	27.8
11	Chickpea (CDC Vanguard)	Spring Wheat	Jul 10	Mid-flower	33.3
12	Pea (CDC Handle)	Spring Wheat	Aug10	Pea maturity	18.8
13	Chickpea (CDC Vanguard)	Durum Wheat	Aug10	Pea maturity	51.2
14	Lentil (CDC Richlea)	Spring Wheat	Sep 10	Maturity	33.3

Fig. 1. Phylogenetic tree constructed from the elongation factor 1α (EF-1 alpha) dataset showing the clustering of F. redolens, OTU 27 and OTU 18 (▴) sequences. F. hostae and F. oxysporum f. sp. asparagi sequences retrieved from GenBank. Bootstrap values from 1000 replicates are indicated above the nodes.

Table 2.   Disease scoring index used in the pathogenicity test

Health status of plants
No visible symptom	0
Limited visible lesions or discoloration on root, chlorosis in leaves with normal plant size	1
Extended lesions or discoloration on root and/or shoot with < 50% reduction in plant size	2
Severe lesion or discoloration on root and/or shoot with > 50% reduction in plant size	3
Dead plant	4

Fig. 2. The effect of inoculation with F. redolens (SMCD 2402) on pea: non-inoculated (left) and inoculated (right).

Fig. 3. Comparative pathogenicity of F. redolens, F. graminearum and C. sativus on chickpea, durum wheat and pea. Disease severity rating is based on the 0–4 scale described in Table 2. Least square means with the same letter are not significantly different at P < 0.05 (n = 6).