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Genetics and resistance/Génétique et résistance

Genetic structure and variation in aggressiveness in Fusarium virguliforme in the Midwest United States

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Pages 83-97 | Accepted 02 Feb 2012, Published online: 27 Apr 2012

Figures & data

Table 1.  Origin, year of collection, genotype classes obtained from analysis of RAPD and (CAT)5 markers, and genetic subgroups of Fusarium virguliforme isolates used in this study

Fig. 1. Dendrogram showing relatedness of genotypes of Fusarium virguliforme isolates from Iowa, Minnesota, and Illinois based on combined (CAT)5 and RAPD binary data and cluster analysis (unweighted paired group method with arithmetic means, UPGMA). Roman numerals at branch ends indicate the different genotypes (I to XXV), and numbers within parentheses represent the number of isolates within each genotype. The numbers on the branches represent bootstrap support values greater than 50%.

Fig. 1. Dendrogram showing relatedness of genotypes of Fusarium virguliforme isolates from Iowa, Minnesota, and Illinois based on combined (CAT)5 and RAPD binary data and cluster analysis (unweighted paired group method with arithmetic means, UPGMA). Roman numerals at branch ends indicate the different genotypes (I to XXV), and numbers within parentheses represent the number of isolates within each genotype. The numbers on the branches represent bootstrap support values greater than 50%.

Table 2.  Analysis of variance on root rot severity and area under disease progress curve (AUDPC) for foliar symptom severity of soybean sudden death syndrome on plants grown in infested greenhouse soil or exposed to culture filtrates of Fusarium virguliforme in two experimental trials

Fig. 2. Area under the disease progress curve (AUDPC) for foliar symptom severity on soybean ‘Spencer’ inoculated with 30 Fusarium virguliforme isolates and incubated for 30 days under greenhouse conditions in (a) trial 1 and (b) trial 2.

Fig. 2. Area under the disease progress curve (AUDPC) for foliar symptom severity on soybean ‘Spencer’ inoculated with 30 Fusarium virguliforme isolates and incubated for 30 days under greenhouse conditions in (a) trial 1 and (b) trial 2.

Fig. 3. Root rot severity on soybean plants ‘Spencer’ inoculated with 30 Fusarium virguliforme isolates and incubated for 30 days under greenhouse conditions in (a) trial 1 and (b) trial 2. Per cent root rot was evaluated at the end of the experiment and was calculated as the percentage of necrotic root area.

Fig. 3. Root rot severity on soybean plants ‘Spencer’ inoculated with 30 Fusarium virguliforme isolates and incubated for 30 days under greenhouse conditions in (a) trial 1 and (b) trial 2. Per cent root rot was evaluated at the end of the experiment and was calculated as the percentage of necrotic root area.

Table 3.  Pearson's correlation coefficient values and significance for Fusarium virguliforme inoculum density in potted mix at the end of the experiment, foliar severity symptoms (area under disease progress curve, AUDPC), root dry weight, shoot length, shoot weight and root rot severity ratings of soybean ‘Spencer’ in trials 1 and 2 of the experiment

Fig. 4. Area under the disease progress curve (AUDPC) for foliar symptom severity on soybean ‘Spencer’ with roots immersed in cell-free culture filtrates of 30 Fusarium virguliforme isolates and incubated for 25 days in a growth chamber. (a) trial 1 and (b) trial 2.

Fig. 4. Area under the disease progress curve (AUDPC) for foliar symptom severity on soybean ‘Spencer’ with roots immersed in cell-free culture filtrates of 30 Fusarium virguliforme isolates and incubated for 25 days in a growth chamber. (a) trial 1 and (b) trial 2.

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