Genetic diversity of Cercospora sojina revealed by amplified fragment length polymorphism markers

Figures & data

Table 1.  Collection of 62 Cercospora sojina isolates used in the genetic diversity study

Isolate	Origin	Isolate	Origin	Isolate	Origin
S1	Georgia	S39	Alabama	S99	Arkansas
S3	Georgia	S40	Georgia	S100	Arkansas
S5	Georgia	S41	Brazil	S102	Mississippi
S6	Georgia	S42	Brazil	S106	Mississippi
S7	Georgia	S43	Brazil	S108	Mississippi
S8	Georgia	S48	Brazil	S111	Louisiana
S9	Georgia	S49	Brazil	S113	Louisiana
S10	Georgia	S51	Brazil	S114	Louisiana
S11	Georgia	S52	Brazil	S115	Mississippi
S12	Georgia	S56	Brazil	S116	Mississippi
S13	Georgia	S58	Brazil	S119	Mississippi
S14	Georgia	S59	Brazil	S120	Mississippi
S16	Georgia	S63	Nigeria	S121	Mississippi
S18	Georgia	S81	Mississippi	S122	Mississippi
S20	China	S82	Louisiana	S123	Iowa
S21	China	S83	Louisiana	S124	Iowa
S22	China	S86	Mississippi	S126	Wisconsin
S23	China	S92	Mississippi	S127	Illinois
S24	China	S95	Mississippi	S128	South Carolina
S25	China	S96	Arkansas	S130	South Carolina
S26	China	S97	Arkansas

Table 2.  Amplified fragment length polymorphism (AFLP) primers used to assess the genetic diversity among Cercospora sojina isolates

Primer combination	Polymorphic markers (no.)
E-AAT, M-CAA	4
E-ATT, M-CAA	8
E-AAT, M-CAC	4
E-ATT, M-CAC	7
E-ATT, M-CAG	7
E-ATT, M-CTG	1
E-ACT, M-CAC	6
E-AAC, M-CAC	3
Total	40

Fig. 1. Phenogram based on simple matching similarity coefficients among 62 Cercospora sojina isolates collected from four countries. The seven clusters are indicated by Roman numerals. The last letter of the isolate name represents the country where the isolate was collected; U: United States; B: Brazil, C: China; N: Nigeria. The cophenetic correlation coefficient r = 0.80.

Table 3.  Geographic distribution of the Cercospora sojina isolates among the seven sub-clusters based on amplified fragment length polymorphism (AFLP) markers

Sub-cluster	Midwestern U.S. (no.)^a	Southern U.S. (no.)^b	Brazil (no.)	China (no.)	Nigeria (no.)
1	2	11	2	-	-
2	-	6	3	2	-
3	-	2	-	1	-
4	1	10	3	2	-
5	1	6	2	-	-
6	-	4	-	2	1
7	-	1	-	-	-
^aMidwestern states included Illinois, Iowa and Wisconsin.
^bSouthern states included Alabama, Arkansas, Georgia, Louisiana, Mississippi and South Carolina.