Figures & data
Fig. 1. Detection of Switchgrass mosaic virus in individual leafhoppers. Panel A: RT–PCR using B287 primer for RT and B88 and B89 primer pairs for PCR. Panel B: RT–PCR using B287 primer for RT and B305 and B89 for PCR. M = GeneRuler 1Kb plus (Fermentas); Lanes 1–5: Fleximiana atlantica (FA); Lanes 6–10: Graminella morhi (GM); Lanes 11–15: G. aureovitatta (GA); Lanes 16–20: Myndus ovatus (MO); Lane 16: Switchgrass leaf from plant on which FA obtained directly from the field were maintained; Lane 17: Switchgrass leaf from plant on which FA obtained directly from the field were maintained; Lane 18: Switchgrass leaf from plant on which GA obtained directly from the field were maintained; Lane 19: Switchgrass leaf from plant on which GM obtained directly from the field were maintained; Lane 20: leaf from uninfested, SwMV-negative switchgrass plant (negative control). FA, GA, GM were leafhoppers, while GO was a planthopper.
Fig. 3. Transmission of Switchgrass mosaic virus by Graminella aureovittata. A. Healthy: Asymptomatic leaf from plant not exposed to leafhoppers). Infected: Mosaic (yellow streak) leaf symptom after SwMV transmission by G. aureovittata. Both plants were switchgrass var. Cave-in-Rock. B. The other two leafhopper species did not transmit the virus to switchgrass, as their infested plants were both asymptomatic and SwMV-negative.
Fig. 2. Detection of Switchgrass mosaic virus in switchgrass transmitted by Graminella aureovittata. Panel A: RT–PCR using B287 primer for RT and B88 and B89 primer pair for PCR. Panel B: RT–PCR using B287 primer for RT and B308 and B89 for PCR. M = GeneRuler 1Kb plus (Fermentas). PCR products 1–8 from eight switchgrass plants exposed to G. aureovittata, while products 9 and 10 were from two switchgrass plants grown from seeds and maintained in insect-free growth chamber. PCR product 1 was from switchgrass plant with foliage mosaic symptom; others were from asymptomatic leaf tissues. Switchgrass variety used was Cave-in-Rock.
