Ratio of 3-ADON and 15-ADON isolates of Fusarium graminearum recovered from wheat kernels in Manitoba from 2008 to 2012

Figures & data

Table 1. Treatments for controlled environment study inoculating plants with mixtures of 3-acetyldeoxynivalenol (ADON) and 15-ADON chemotypes of Fusarium graminearum.

	3-ADON isolates		15-ADON isolates
Treatment mixtures	M9-04-6	M6-04-4	M1-04-1	M8-04-3
NRRL†	37480	37456	37424	37466
1	x		x
2		x	x
3	x			x
4		x		x
5	x	x	x	x
6	Water control

Note: † ARS Culture Collection number.

Fig. 1 Average monthly weather variables for June and July at Carman, MB for 2008 and 2009.

Table 2. Recovery of 3-acetyldeoxynivalenol (ADON) and 15-ADON isolates of Fusarium graminearum from grain harvested from plants after inoculation with mixtures of chemotypes in a 1:1 ratio in different environments: Carman field plots 2008, Carman field plots 2009, and Greenhouse, and Growth Room experiments with ‘CDC Teal’ wheat.

Carman Field 2008		Observed	Observed		Chi square	Chi square
		3-ADON	15-ADON	Total	value	probability
Line	Resistance to FHB
AC Barrie	I	38†	18	56	7.1429	0.0075
93FHB37	R	42	14	56	14.0000	0.0002
Superb	S	31	25	56	0.6429	0.4227
5602HR	MR	28	28	56	0.0000	1.0000
CDC Teal	S	34	22	56	2.5714	0.1088
Total		173	107	280	15.5571	0.0001
Carman Field 2009
AC Barrie	I	36	18	54	6.0000	0.0143
93FHB37	R	27	20	47	1.0426	0.3072
Superb	S	31	25	56	0.6429	0.4227
5602HR	MR	35	21	56	3.5000	0.0614
CDC Teal	S	26	26	52	0.0000	1.0000
Total		155	110	265	7.6415	0.0057
Greenhouse
Treatment mixture	ADON3	ADON15
1	39	67	106	7.3962	0.0065
2	56	47	103	0.7864	0.3752
3	33	63	96	9.3750	0.0022
4	41	74	115	9.4696	0.0021
5	25	65	90	17.7778	0.0000
Total	194	316	510	29.1843	0.0000
Growth Room
1	45	41	86	0.1860	0.6662
2	49	52	101	0.0891	0.7653
3	23	69	92	23.0000	0.0000
4	15	92	107	55.4112	0.0000
5	46	54	100	0.6400	0.4237
Total	308	486	243	260.3868	0.0000

Note: † Values in bold indicate a significant deviation from the expected 1:1 ratio.

Fig. 2 Recovery of 3-acetyldeoxynivalenol (ADON) and 15-ADON isolates of Fusarium graminearum from Glenlea MB, fusarium head blight nurseries inoculated with both chemotypes from 2008 to 2012. Results for 2008 and 2011 did not fit a 1:1 ratio.

Table 3. Recovery of 3-acetyl deoxynivalenol (ADON) and 15-ADON isolates of Fusarium graminearum recovered from wheat kernels in inoculated fusarium head blight nurseries at Glenlea, Manitoba from 2008–2012.

		Observed	Observed		Chi square	Chi square
Cv	Year	ADON3	ADON15	Total	value	probability
‘5602HR’	2008	31†	9	40	12.1	0.0005
(MR) ‡	2009	21	19	40	0.1	0.7518
	2010	19	21	40	0.1	0.7518
	2011	20	20	40	0	1.0000
	2012	27	13	40	4.9	0.0269
‘93FHB37’	2008	31	9	40	12.1	0.0005
(R)	2009	24	16	40	1.6	0.2059
	2010	22	18	40	0.4	0.5271
	2011	20	20	40	0	1.0000
	2012	28	12	40	6.4	0.0114
‘AC Morse’	2008	34	6	40	19.6	0.0000
(S)	2009	19	21	40	0.1	0.7518
	2010	21	19	40	0.1	0.7518
	2011	16	24	40	1.6	0.2059
	2012	11	29	40	8.10	0.0044
‘AC Snowbird’	2008	34	6	40	19.60	0.0000
(I)	2009	18	22	40	0.4	0.5271
	2010	21	19	40	0.1	0.7518
	2011	10	30	40	10.00	0.0016
	2012	15	25	40	2.50	0.1138
‘AC Vista’	2008	31	9	40	12.1	0.0005
(S)	2009	22	18	40	0.4	0.5271
	2010	20	20	40	0	1.0000
	2011	19	21	40	0.1	0.7518
	2012	15	25	40	2.5	0.1138
‘Roblin’	2008	28	12	40	6.4	0.0114
(S)	2009	29	11	40	8.1	0.0044
	2010	19	21	40	0.1	0.7518
	2011	18	22	40	0.4	0.5271
	2012	20	20	40	0	1.0000

Notes: † Values in bold indicate a significant deviation from the expected 1:1 ratio.

‡ Resistance to FHB Resistant, moderately resistant, intermediate or susceptible.

Fig. 3 Average monthly weather variables for Morris, MB from 2008 to 2012: a. maximum and minimum temperatures, b. precipitation.

Table 4. Recovery of 3-acetyldeoxynivalenol (ADON) and 15-ADON isolates of Fusarium graminearum after incubation of inoculated wheat plants at 20, 24 and 28 °C.

Cv			Observed	Observed
20°C	Resistance to FHB	Repetition	3ADON	15ADON	Total	Chi square value	Chi square probability
5602 HR	MR	1	4	36†	40	25.60	0.0000
ACSnowbird	I	1	11	27	38	6.74	0.0094
93FHB37	R	1	−‡	–	0	−	−
AC Vista	S	1	13	27	40	4.90	0.0269
Roblin	S	1	19	21	40	0.10	0.7518
AC Morse	S	1	12	28	40	6.40	0.0114
5602 HR	MR	2	20	20	40	0.00	1.0000
ACSnowbird	I	2	19	21	40	0.10	0.7518
93FHB38	R	2	14	26	40	3.60	0.0578
AC Vista	S	2	8	32	40	14.40	0.0001
Roblin	S	2	11	29	40	8.10	0.0044
AC Morse	S	2	11	29	40	8.10	0.0044
24°C
5602 HR	MR	1	26	6	32	12.50	0.0004
ACSnowbird	I	1	7	23	30	8.53	0.0035
93FHB37	R	1	17	4	21	8.05	0.0046
AC Vista	S	1	17	23	40	0.90	0.3428
Roblin	S	1	8	26	34	9.53	0.0020
AC Morse	S	1	21	11	32	3.13	0.0771
5602 HR	MR	2	21	19	40	0.10	0.7518
ACSnowbird	I	2	32	8	40	14.40	0.0001
93FHB38	R	2	24	16	40	1.60	0.2059
AC Vista	S	2	24	16	40	1.60	0.2059
Roblin	S	2	22	18	40	0.40	0.5271
AC Morse	S	2	26	14	40	3.60	0.0578
28°C
5602 HR	MR	1	13	5	18	3.56	0.0593
ACSnowbird	I	1	34	6	40	19.60	0.0000
93FHB37	R	1	–	–	0	−	−
AC Vista	S	1	26	10	36	7.11	0.0077
Roblin	S	1	20	20	40	0.00	1.0000
AC Morse	S	1	13	7	20	1.80	0.1797
5602 HR	MR	2	20	20	40	0.00	1.0000
ACSnowbird	I	2	32	8	40	14.40	0.0001
93FHB38	R	2	21	19	40	0.10	0.7518
AC Vista	S	2	32	8	40	14.40	0.0001
Roblin	S	2	29	11	40	8.10	0.0044
AC Morse	S	2	22	18	40	0.40	0.5271

Notes: † Values in bold indicate a significant deviation from the expected 1:1 ratio.

‡ No data.