Virulence of Pyrenophora teres populations in western Canada

Figures & data

Table 1. Pathogenic variation of 39 Canadian isolates of Pyrenophora teres f. teres (net form net blotch of barley) on nine barley differentials based on a 1–10 scale. Regular and bold font numbers denote resistant (<5) and susceptible (≥5) phenotypes, respectively.

Isolate	CI5791	CI9820	TR473	Norbert	BT201	Heartland	Steptoe	CI9214	OAC21	Average	Pathotype
MB11	2.0	3.4	3.0	2.8	3.9	2.3	3.4	2.2	2.7	2.8	A01
AB11	2.0	2.3	3.3	2.7	7.3	2.0	2.1	2.5	3.7	3.1	B02
MB10	3.0	3.0	3.7	3.7	9.1	3.7	4.0	4.9	9.5	4.9	C04
AB53	3.0	3.8	2.9	3.8	8.8	3.8	3.0	4.0	9.3	4.7	C04
ABV28	3.0	3.0	2.0	3.0	9.0	3.5	4.0	4.9	8.5	4.5	C04
SK33	2.5	3.2	3.3	2.9	7.9	3.8	3.3	3.3	7.3	4.2	C04
AB04	1.7	1.9	2.0	2.1	9.3	2.8	4.0	3.9	9.0	4.1	C04
MB04	2.5	2.6	2.3	2.4	8.8	2.9	2.8	3.8	8.3	4.0	C04
AB01	2.0	2.7	2.2	2.3	7.4	2.1	2.2	3.9	8.5	3.7	C04
ABV01	3.0	3.0	3.0	3.0	7.0	2.5	3.0	2.0	6.0	3.6	C04
SK52	2.1	1.8	3.8	5.6	6.6	1.5	3.7	1.4	1.5	3.1	C06
SK07	2.1	3.4	2.5	3.7	9.3	2.5	3.6	5.3	9.8	4.7	D04
AB28	2.3	2.6	1.9	2.1	8.5	2.9	2.6	6.8	9.0	4.3	D04
SK08	1.7	1.9	1.5	2.5	9.1	2.3	2.7	5.8	9.2	4.1	D04
AB06	1.5	2.1	2.0	1.7	7.5	2.0	2.7	7.0	8.4	3.9	D04
AB51	4.0	3.6	3.0	3.5	8.3	3.8	7.4	4.0	7.5	5.0	D05
AB35	3.0	2.6	2.4	2.7	8.9	4.2	5.8	4.2	8.7	4.7	D05
SK53	3.1	2.4	4.0	4.9	8.0	2.9	5.0	3.4	8.3	4.7	D05
AB32	1.8	1.5	1.8	1.5	8.3	2.4	8.2	3.2	8.9	4.2	D05
SK03	3.8	3.3	6.6	7.0	8.3	2.5	3.8	3.3	2.7	4.6	D09
SK24	2.0	2.4	5.6	4.0	8.0	2.5	5.5	1.5	2.2	3.7	D10
ABV18	3.0	3.0	3.0	3.5	9.0	3.0	8.0	5.5	5.5	4.8	E02
MB15	1.8	2.2	2.0	2.0	9.0	2.4	6.3	5.8	9.0	4.5	E02
AB16	3.5	3.3	3.3	3.0	9.3	7.8	3.3	7.3	9.3	5.5	E03
AB38	2.5	2.1	2.2	2.9	9.3	5.8	6.5	3.0	9.1	4.8	E07
MB01	2.4	2.6	7.5	7.3	7.5	2.1	2.9	4.9	8.5	5.1	E09
SK26	2.9	2.8	7.8	8.1	7.7	1.8	2.3	2.6	8.1	4.9	E09
MB03	2.0	2.3	8.3	7.1	7.8	2.4	3.0	2.6	7.4	4.8	E09
AB12	2.3	2.1	7.3	7.7	8.1	1.3	2.7	1.3	7.8	4.5	E09
SK16	3.0	2.9	5.7	7.1	6.4	1.7	4.5	2.3	6.0	4.4	E09
MB06	2.3	2.2	5.7	6.6	6.3	1.4	3.8	2.0	8.3	4.3	E09
SK05	2.1	2.2	5.6	6.2	7.3	1.0	2.8	2.6	7.8	4.2	E09
AB48	2.0	2.0	6.0	7.1	6.9	1.1	2.1	1.3	7.8	4.0	E09
SK01	2.1	1.8	6.0	5.8	6.4	1.2	1.8	1.0	7.0	3.7	E09
WRS102	3.4	3.3	3.5	3.9	9.3	5.1	7.3	4.5	9.5	5.5	E10
MB02	3.7	3.3	7.8	7.8	8.8	3.6	3.8	5.6	4.3	5.4	E11
MB05	3.0	2.8	3.3	4.3	9.0	6.2	7.6	7.6	9.3	5.9	F01
AB34	2.8	2.7	3.5	3.6	9.0	6.3	7.6	6.9	9.7	5.8	F01
SK41	2.9	3.7	7.3	6.9	6.2	2.8	8.2	3.3	8.7	5.6	F06
MB14	5.3	6.7	3.9	6.5	9.0	6.2	6.0	5.8	9.3	6.5	I03
Average	2.6	2.8	4.1	4.4	8.0	3.0	4.3	3.9	7.5	4.5	N/A

Fig. 1 Pathogenic similarity of 39 Pyrenophora teres f. teres (net form net blotch of barley) isolates recovered from barley crops in western Canada; two pathotype groups, highlighted in boxes, were found to be predominant. The dendrogram was produced using the unweighted pair-group method using the arithmetic means (UPGMA) procedure and simple similarity coefficient with NTSYSpc ver. 2.2.

Fig. 2 a, Percentage of western Canadian Pyrenophora teres f. teres isolates virulent on nine barley differential genotypes; BT 201 (97.4%, 38 isolates), ‘OAC 21ʹ (84.6%, 33 isolates), ‘Norbert’ (35.9%, 14 isolates), TR 473 (33.3%, 13 isolates), ‘Steptoe’ (30.8%, 12 isolates), CI 9214 (28.2%, 11 isolates), ‘Heartland’ (12.8%, 5 isolates), CI 5791 (2.6%, 1 isolate), CI 9820 (2.6%, 1 isolate). b, Percentage of western Canadian Pyrenophora teres f. maculata isolates virulent on 11 barley differential genotypes; ‘Betzes’ (92.6%, 25 isolates), ‘Herta’ (88.9%, 24 isolates), TR 473 (85.2%, 23 isolates), ‘Norbert’ (85.2%, 23 isolates), ‘Bonanza’ (85.2%, 23 isolates), ‘Steptoe’ (81.5%, 22 isolates), CI 9820 (77.8%, 21 isolates), CI 5791 (70.4%, 19 isolates), ‘OAC 21ʹ (55.6%, 15 isolates), ‘Heartland’ (51.9%, 14 isolates), CI 9214 (7.4%, 2 isolates).

Fig. 3 a, Comparison of the main pathotype groups (A–J) found among Pyrenophora teres f. teres (net form net blotch of barley) populations from western Canada in 1985 (Tekauz 1990) versus 2009–2011 (current study). b, Comparison of the main pathotype groups (L–W) found among the Pyrenophora teres f. maculata (spot form net blotch of barley) populations from western Canada in 1985 (Tekauz 1990) versus 2009–2011 (current study).

Table 2. Pathogenic variation of 27 Canadian isolates of Pyrenophora teres f. maculata (spot form net blotch of barley) on 11 barley differentials based on a 1–9 scale. Regular and bold font numbers denote resistant (1–3) and susceptible (>3) phenotypes, respectively.

Isolate	CI5791	CI9820	TR473	Norbert	Heartland	Steptoe	CI9214	OAC21	Bonanza	Herta	Betzes	Average	Pathotype
AB57	1.90	1.80	2.40	2.40	1.40	1.80	1.70	3.00	2.60	2.50	3.00	2.20	L01
AB68	2.50	2.50	2.20	2.10	2.00	2.60	2.30	2.10	2.80	2.60	2.90	2.40	L01
AB82	2.40	2.80	2.80	2.60	1.80	3.80	1.60	2.40	2.80	3.00	4.00	2.70	N01
MB32	3.00	3.00	4.00	4.30	2.50	3.00	2.50	3.00	3.00	4.00	4.00	3.30	P03
AB61	3.00	3.00	3.00	3.00	2.80	3.00	3.00	3.00	5.00	4.00	5.50	3.50	O01
WRS857	3.70	3.00	3.90	3.50	4.00	3.00	4.00	3.00	3.00	3.80	5.00	3.60	T06
MB26	3.90	4.80	4.80	5.30	3.00	4.80	3.00	3.00	4.00	4.50	5.00	4.20	T01
SK88	3.00	4.00	4.50	5.20	2.50	5.00	3.00	3.00	4.40	6.80	6.50	4.40	S01
SK87	3.00	3.00	5.80	6.50	4.50	4.50	2.50	4.00	6.30	5.00	5.00	4.60	T04
SK69	4.00	4.30	4.80	6.80	3.00	5.40	2.80	2.80	5.50	5.60	5.10	4.60	T01
MB22	4.00	4.00	6.00	6.50	3.00	4.50	4.00	3.00	5.00	5.00	6.00	4.60	U04
AB58	3.00	5.20	6.10	5.30	3.80	4.90	3.00	3.00	5.60	6.90	4.90	4.70	U02
AB74	4.50	5.40	6.00	4.80	3.00	5.50	3.00	4.00	4.60	6.80	5.70	4.80	U01
SK73	4.30	5.50	5.30	5.70	2.90	5.10	2.50	5.30	6.70	4.10	6.00	4.90	U01
SKV08	4.00	5.00	7.00	6.00	3.00	5.00	2.00	3.00	5.50	7.00	6.00	4.90	T01
SK100	4.00	5.00	7.00	7.00	3.00	4.00	3.00	3.00	5.00	6.00	7.00	4.90	T01
MB23	3.60	5.00	5.80	7.10	3.60	3.00	2.70	3.70	6.20	7.10	6.30	4.90	U03
MB24	4.80	5.00	5.80	6.80	4.30	5.10	3.00	3.90	5.40	5.20	5.30	5.00	V01
SK64	5.00	5.00	6.00	5.60	4.00	3.70	3.00	3.80	6.30	6.40	6.80	5.10	V01
AB79	4.00	4.80	5.80	6.10	3.80	5.00	2.90	3.90	5.80	6.80	6.70	5.10	V01
MB16	4.80	5.00	6.40	6.30	4.50	4.50	2.50	4.50	6.00	6.80	5.30	5.10	V01
SKV12	4.00	5.00	7.50	8.00	4.00	5.00	2.00	4.00	5.50	7.00	6.00	5.30	V01
ABV14	5.00	5.00	6.50	7.50	4.30	4.50	3.00	3.80	4.00	8.50	8.00	5.50	V01
SKV10	4.50	4.50	8.00	8.00	4.50	4.50	3.00	4.00	6.00	8.00	6.50	5.60	V01
MB21	4.00	4.00	7.50	8.00	4.50	6.00	3.00	4.50	6.50	7.00	7.00	5.60	V01
SK60	4.50	5.50	6.00	5.50	5.30	5.40	4.50	4.50	7.50	7.20	6.80	5.70	W01
MBV37	5.50	5.50	7.30	7.50	4.00	4.00	3.00	4.00	5.50	9.00	8.30	5.80	V01
MBV25	5.00	6.00	8.00	7.30	5.40	4.00	3.00	4.00	8.00	9.00	7.50	6.10	V01
Average	3.90	4.40	5.60	5.70	3.50	4.30	2.80	3.50	5.20	5.90	5.80	4.60	N/A

Fig. 4 Pathogenic similarity of Pyrenophora teres f. maculata (spot form net blotch of barley) isolates recovered from barley crops in western Canada; two pathotype groups, highlighted in boxes, were found to be predominant. The dendrogram was produced using the unweighted pair-group method using the arithmetic means (UPGMA) procedure and simple similarity coefficient with NTSYSpc ver. 2.2.

Fig. 5 Pathogenic similarity of 36 P. teres f. teres (Ptt) and 21 P. teres f. maculata (Ptm) isolates collected from western Canada. Isolates clustered in two distinct groups with 35 Ptt and 6 Ptm isolates in the first group and 1 Ptt and 15 Ptm isolates in the second group. The dendrogram was produced using the unweighted pair-group method using the arithmetic means (UPGMA) procedure and simple similarity coefficient with NTSYSpc ver. 2.2.

Fig. 6 Genetic similarity of 36 P. teres f. teres (Ptt) and 21 P. teres f. maculata (Ptm) isolates collected from western Canada. Cluster analysis revealed that all isolates clustered in two distinct divergent groups conforming to either Ptt or Ptm, with no intermediate cluster between the two forms. The dendrogram was produced using the unweighted pair-group method using the arithmetic means (UPGMA) procedure and simple similarity coefficient with NTSYSpc ver. 2.2.

Tekauz A. 1990. Characterization and distribution of pathogenic variation in Pyrenophora teres f. teres and P. teres f. maculata from Western Canada. Can J Plant Pathol. 12:141–148.

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