First report of tomato leaf spot caused by Fusarium proliferatum in China

Figures & data

Fig. 1 (Colour online) Leaf spot of tomato caused by F. proliferatum. (a) Necrotic lesions on tomato leaf in commercial tomato greenhouse; (b) 7-day-old colony of F. proliferatum growing on potato dextrose agar at 20°C; (c) Fully grown colony with sclerotia; (d) Macroconidia and microconidia of the fungus formed on PDA; (e), (f) Necrotic spots on tomato leaf and stem infected with F. proliferatum 4 days after artificial inoculation.

Table 1. Sequence identities for the five genes found by a BLAST search in the GenBank database.

Gene name	Fragment size (bp)	Closest relative	Identity (%)^a	Accession no.^b
TEF	704	F. proliferatum strain CBS 131574 (JX118983)	100	KT218533
BT	1644	F. proliferatum NRRL 31071 (AF291055)	99	KT218534
FUM1	646	F. proliferatum strain ITEM 2287 (JX118983)	98	KU187896
H3	487	F. proliferatum NRRL 31071 (AF291059)	100	KU187897
Cal	497	F. proliferatum NRRL 31071 (AF291057)	100	KU187898

^aPercentage of identical nucleotides in the sequences amplified with five sets of specific primers in F. proliferatum and the sequences of the closest relatives found by a BLAST search in the GenBank database.

^bAccession numbers of the sequences that have been deposited in GenBank.

Fig. 2 Phylogenetic tree constructed with sequences of partial translation elongation factor-1α (TEF). Gene sequences show the closest known relatives of F. proliferatum, inferred by the neighbour-joining method and reference sequences retrieved from GenBank database. The per cent bootstrap support values (1000 replications; ≥70%) are shown in the branches. Bar = number of nucleotide substitutions per site.