Identification of Plum pox virus causing sharka disease on peach (Prunus persica L.) in Mexico

Figures & data

Fig. 1 (Colour online) Plum pox virus symptoms on a peach tree in orchards in Aguascalientes, Mexico.

Table 1. Number of peach leaf samples collected, and those positive for Plum pox virus by dot blot or RT-PCR.

Orchard	Number of samples	Dot blot positive	RT-PCR positive
1	39	4	2
2	30	2	0
3	48	2	1
4	38	2	2
5	107	15	0
Total	262	25	5

Fig. 2 (Colour online) (a) Dot blot analysis performed on samples collected from different orchards for Potyvirus scrutiny. 1/a: negative (healthy plants), 2/a: positive for Potyvirus (Papaya ringspot virus), 2/b to 8/g: samples from apparently infected peach trees. (b) RT-PCR products obtained with consensus oligos for Potyvirus. In all cases a band of 260 bp was obtained. Lanes: (1) DNA ladder, (2) positive control, and (3–7) samples of infected peach trees. (c) RsaI restriction profile in the CP region representative of PPV-D genotype by RFLP analyses. Lanes: (1) DNA ladder, (2) PPV genotype D, showing the amplicon of ~450 bp and two fragments of ~300 and ~150 bp, (3) complete digestion with RsaI.

Table 2. Pathogenicity test on three plant species using Plum pox virus inoculum and percentage of inoculation success.

Orchard	Samples	Number of symptomatic plants/number of inoculated plants
		N. benthamiana	C. amaranthicolor	S. oleraceus
5*	0–4	0/5	0/2	0/2
1	4–4-12	5/5	0/2	2/2
1	4–13-9	5/5	0/2	2/2
4	6–5-38	5/5	0/2	2/2
4	6–3-5	5/5	0/2	1/2
3	5–11-8	5/5	0/2	1/2

* Negative controls.

Fig. 3 (Colour online) Pathogenicity test on S. oleraceus showing necrotic leaf spots on leaves.