A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus

Figures & data

Fig. 1 (Colour online) Optimization of the reaction conditions in RT-LAMP for PeVYV detection. (a) Different incubation temperature; (b) Different incubation duration; (c) Sensitivity of RT-LAMP using serial (10-fold) dilutions of total RNA of PeVYV infected pepper; (d) Sensitivity of RT-PCR using serial (10-fold) dilutions of total RNA of PeVYV infected pepper; (e) Specificity using total RNA of four other viruses and PeVYV infected pepper; PLRV, Potato leafroll virus; PYV, Potato yellow virus; BCV, Beet chlorosis virus; TVDV, Tobacco vein distorting virus; M indicates DNA ladder; NTC indicates the non-template control.

Table 1. Detection of PeVYV infection in field-collected samples using the optimized RT-LAMP or conventional RT-PCR.

Sampling site	No. of samples tested	No. of positive samples by RT-LAMP	No. of positive samples by RT-PCR
Hunan Province	11	2	2
Guangxi Province	16	9	9