An efficient CAPS marker for bacterial-blight resistance gene xa25 in rice

Figures & data

Fig. 1 Promoter sequences alignment of xa25 from ‘Minghui63' and Xa25 from ‘Zhenshan97'. The sequences of recognition site of MwoI are underlined. The −40 (G to T) substitution is boxed. The sequences of promoters are used by upper-case letters, and of 5ʹ UTRs are used by lower-case letters. Arrows indicate the location of the PCR primers for marker xa25-M.

Fig. 2 Validation of marker xa25-M with rice cultivars containing the xa25 or Xa25 gene.

Fig. 3 Validation of marker xa25-M in F₂ population derived from a cross between Minghui63 and IR24.

Table 1. Phenotype of nine Chinese rice landraces genotyped with xa25-M marker.

No.	Landraces	Phenotype^a	Genotype^b
1	Yang-ke-nuo	R	R
2	Gui-hua-huang	R	R
3	Bai-ke-hua-luo	S	S
4	Cheng-nong-shui-jing-mi	R	R
5	Li-xin-jing	R	R
6	Nan-xiong-zao-you	S	S
7	Qi-tou-bai-gu	S	S
8	Zi-mi	S	S
9	Xiang-gu	S	S

^aPhenotype of BB resistance by inoculation with Xoo PXO339 as resistant (R), moderate resistant (MR) and susceptible (S);

^bGenotype of xa25-M marker as homozygous resistant (R), homozygous susceptible (S).