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Virology/Virologie

Charlock mustard (Sinapis arvensis): a weed reservoir for begomoviruses and associated betasatellite in Jordan

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Pages 325-333 | Accepted 06 Jul 2017, Published online: 08 Aug 2017

Figures & data

Fig. 1 (Colour online) A plant of Charlock mustard (Sinapis arvensis) showing severe leaf curling symptoms due to natural infection by a complex of begomoviruses.

Fig. 1 (Colour online) A plant of Charlock mustard (Sinapis arvensis) showing severe leaf curling symptoms due to natural infection by a complex of begomoviruses.

Table 1. Genome characteristics of begomoviruses reported in this study.

Fig. 2 (Colour online) Colour-coded pairwise identity matrix generated from 23 complete DNA-A genome segment of begomoviruses. The analyses include SLCV-[JO:Sin:11] (a) and TYLCSV-[JO:Sin:11] (b) from this study (in red) and the corresponding published sequences of selected begomoviruses. The matrix was generated using the program Sequence Demarcation Tool (SDT v.1.2; http://web.cbio.uct.ac.za/), and each coloured cell represents a percentage identity score for two sequences. Coloured keys indicating the correspondence between pairwise identities and the colours displayed in the matrix are presented.

Fig. 2 (Colour online) Colour-coded pairwise identity matrix generated from 23 complete DNA-A genome segment of begomoviruses. The analyses include SLCV-[JO:Sin:11] (a) and TYLCSV-[JO:Sin:11] (b) from this study (in red) and the corresponding published sequences of selected begomoviruses. The matrix was generated using the program Sequence Demarcation Tool (SDT v.1.2; http://web.cbio.uct.ac.za/), and each coloured cell represents a percentage identity score for two sequences. Coloured keys indicating the correspondence between pairwise identities and the colours displayed in the matrix are presented.

Fig. 3 Maximum likelihood phylogenetic tree based on the complete DNA-A sequence of TYLCSV-[JO:Sin:11], TYLCV-[JO:Sin:11], WmCSV-[JO:Sin:11] and SLCV-[JO:Sin:11] (arrows) and sequences of selected begomoviruses available in GenBank. Support for nodes in a bootstrap analysis with 1000 replications is shown. The tree was generated using the Maximum likelihood method in MEGA 6.06. An isolate of Beet curly top virus (BCTV-[US:Sp3]) AY548948 was included as outgroup.

Fig. 3 Maximum likelihood phylogenetic tree based on the complete DNA-A sequence of TYLCSV-[JO:Sin:11], TYLCV-[JO:Sin:11], WmCSV-[JO:Sin:11] and SLCV-[JO:Sin:11] (arrows) and sequences of selected begomoviruses available in GenBank. Support for nodes in a bootstrap analysis with 1000 replications is shown. The tree was generated using the Maximum likelihood method in MEGA 6.06. An isolate of Beet curly top virus (BCTV-[US:Sp3]) AY548948 was included as outgroup.
Supplemental material

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