First report of Sclerotinia subarctica in France detected with a rapid PCR-based test

Figures & data

Table 1. Origin of the 969 Sclerotinia spp. isolates tested for possible identification as S. subarctica.

Field crops	Number of isolates	Origin and location^a
Bean	26	Diseased plant (29, 33, 40, 56)
	3	Air (33)
Canola	11	Diseased plant (10, 17, 18, 21, 47, 57, 77, 78, 82)
Carrot	131	Diseased plant (29, 33, 40, 50, 66, 84, 86)
	495	Air (17, 33, 50, 86)
Lettuce	27	Diseased plant (49, 50, 66, 84)
Melon	81	Diseased plant (17, 81, 82, 86)
	38	Air (17, 33)
	8	Soil (17)
Witloof chicory	28	Diseased plant (59, 60, 62, 80)
	114	Airborne (62)
Other plants (endive, flax, hemp, pea, tomato, weed)	7	Diseased plant (10, 29, 40, 78)
Total	969

^aNumbers in parentheses refer to the official codes of French districts (ISO 3166; https://www.iso.org/obp/ui/#iso:code:3166:FR).

Fig. 1 Electrophoretic gel showing PCR products after amplification of microsatellite loci scsp1-ms-01 and scsp1-ms-08 (Winton et al., 2007), on Sclerotinia sp. and Botrytis cinerea isolates.

Table 2. Polymorphism of five microsatellite markers among 968 isolates of Sclerotinia sp. and one isolate identified as S. subarctica.

	Number of alleles		Size range (bp)
Microsatellite locus	968 isolates	SS_2428	968 isolates	SS_2428
13–2	13	0	274–357	–
17–3	8	1	336–360	369
110–4	6	0	366–386	–
114–4	19	1	335–411	315
55–4	10	0	142–222	–

Microsatellite markers designed by Sirjusingh & Kohn (2001).

Winton LM, Krohn AL, Leiner RH. 2007. Microsatellite markers for Sclerotinia subarctica nom. prov., a new vegetable pathogen of the High North. Mol Ecol Notes. 7:1077–1079.

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Sirjusingh C, Kohn LM. 2001. Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum. Mol Ecol Notes. 1:267–269.

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