Figures & data
Fig. 1 (Colour online) Mycelial growth rate of P. capsici isolates MACO1 and MAL2 on different media. Error bars indicate the standard error (SE) of five replicates.
Fig. 2 (Colour online) Morphological characteristics of Phytophthora capsici isolated from hot pepper plants. a, d, colony morphology of isolates MACO1 and MAL2 on PDA after 5 days. b, e, colony morphology of isolates MACO1 and MAL2 on CMA after 3 days. c, f, colony morphology of isolates MACO1 and MAL2 on V8A after 5 days. g, sporangia (Sp) and zoospores (Zs) from isolate MACO1. h, sporangia (Sp) from isolate MAL2. i, antheridium (At) and oogonium (Og) produced from the pairing of isolates MACO1 and MAL2 on CMA for 20 days. Bars: a–f = 1 cm, g–i = 10 µm.
Fig. 3 (Colour online) Gel electrophoresis of PCR products amplified from P. capsici isolates MACO1 and MAL2 using Phytophthora genus-specific (COX1 & COX2) and ITS (ITS4 & 6) primer pairs. Lane 1: 100 bp marker (Promega Corporation, USA), 2: MACO1 (COX gene), 3: MAL2 (COX gene), 4: blank sample, 5: MACO1 (ITS), 6: MAL2 (ITS).
Fig. 4 (Colour online) Hot pepper plants showing wilting and collar rot symptoms of Phytophthora capsici. a, b, symptoms on hot pepper plants in the field. c, healthy seedling; d, diseased seedling at 3 days after inoculation; e, diseased seedling at 6 days after inoculation; f, dead seedling at 10 days after inoculation. Bar = 2 cm.
