Incidence and molecular characterization of a 16SrI-B phytoplasma strain associated with Vitis vinifera leaf yellowing and reddening in the west of Iran

Figures & data

Fig. 1 (Colour online) Symptoms of (A) leaf curling (B) reddening (C) yellowing (D) little leaves in Vitis vinifera infected plant in Kurdistan (Iran).

Fig. 2 Polyacrylamide gel showing RFLP profiles of 16S rDNA amplified by nested PCR using P1/P7 followed by R16F2n/R16R2 primer pairs from KVvY (GenBank accession number MH638316). PCR products were digested by AluI, HhaI, HpaII, RsaI, TaqI, KpnI, HaeIII, Sau3AI restriction enzymes. M, 100 bp DNA ladder.

Fig. 3 Neighbour-joining phylogenetic tree based on the 1.2 kb 16S rDNA sequence of KVvY (GenBank accession number MH638316) and those of selected phytoplasma strains from GenBank. A. laidlawii was used as an outgroup. GenBank accession numbers are shown on the right of phytoplasma strains. Numbers on branches are bootstrap values of 1000 replicates.

Fig. 4 Virtual RFLP profiles from the R16F2n/R16R2 sequence of the VivY phytoplasma (GenBank accession number MH638316) using 17 restriction endonucleases with iPhyClassifier (Zhao et al., 2009).

Zhao Y, Wei W, Lee I-M, Shao J, Suo X, Davis RE. 2009. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol. 59:2582–2593.

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