Heterobasidion species detected using High Throughput Sequencing (HTS) methods on British Columbia nursery plants

Figures & data

Table 1. Asymptomatic live plant samples collected in 2015 in British Columbia.

Local Plants (L)
Sample #	Species/cultivar
15-1L	Rhododendron ‘Percy Wiseman’
15-2L	Rosa sp.
15-3L	Rosa ‘Anna’s Promise’
15-4L	Rosa ‘Gold Medal’
15-8L	Hydrangea macrophylla ‘White Delight’
15-9L	Hydrangea macrophylla ‘Early Blue’
15-10L	Hydrangea macrophylla ‘Pink Beauty’
15-11L	Buxus sempervirens ‘Variegata’
15-12L	Buxus sempervirens ‘Common boxwood’
15-15L	Chrysanthemum ‘Echo Bronze’
15-16L	Chrysanthemum ‘Eureka Yellow’
Import Plants (IM)
Sample #	Species/cultivar
15-1IM	Chrysanthemum ‘Junea’
15-2IM	Chrysanthemum ‘Junea’
15-3IM	Rosa ‘Meilland’
15-4IM	Rosa ‘Paint the town’
15-5IM	Rosa ‘Coral cove’
15-6IM	Rosa ‘Yellow submarine’
15-7IM)	Hydrangea macrophylla
15-8IM	Hydrangea paniculata ‘Limelight’
15-9IM	Hydrangea paniculata ‘Quick Fire’
15-10IM	Rhododendron ‘Percy Wiseman’
15-11IM	Rhododendron ‘PJM Elite’
15-12IM	Buxus ‘Green Velvet’
15-13IM	Buxus sp.
15-14IM	Buxus ‘Green Mound
15-15IM	Buxus ‘Green Mound

Table 2. Putative identification of the 20 most common fungi and species of the genus Heterobasidion present on BC nursery plants collections. Number of DNA reads, plant sample number (1–15) and origin (L: locally grown plants, IM: imported plants grown in BC nurseries) are given for 15 plants samples.

Otu	# of reads	Putative Identification	Closest Genbank Accession #	% similarity	15-1L	15-9L	15-11L	15-12L	15-15L	15-16L	15-7IM	15-8IM	15-9IM	15-10IM	15-11IM	15-12IM	15-13IM	15-14IM	15-15IM
OTU_1	411 214	Epicoccum nigrum	KT722360	100	18 813	10 897	16 085	24 147	9127	5675	94 077	65 721	36 111	16 726	5051	23 049	22 626	25 524	37 585
OTU_3	67 496	Paraconiothyrium fuckelii	LN808932	100	11 598	3151	7622	17 980	4504	2420	2729	2686	1130	1836	786	2708	1652	5623	1071
OTU_4	11 335	Cladosporium cladosporioides	KU680349	100	985	285	817	1048	540	67	2476	1377	349	442	123	642	511	580	1093
OTU_5	15 265	Alternaria tenuissima	KT758094	100	1541	512	1076	1670	642	320	2821	2093	877	476	167	622	590	756	1102
OTU_9	4201	Aureobasidium pullulans	KT898770	100	385	235	345	336	199	112	578	683	121	208	53	254	183	225	284
OTU_12	2357	Cryptococcus aff. victoriae	KP714646	98,1	45	11	1123	92	627	5	184	43	16	18	8	48	20	42	75
OTU_13	1459	Exobasidium rhododendri	EU784219	96,44	1	1	131	31	105	0	6	6	6	6	5	6	1151	3	1
OTU_18	1243	Exophiala sp. CPC 12 171	EU035420	100	268	86	142	129	75	42	177	103	4	48	4	75	42	19	29
OTU_19	4379	Fusarium avenaceum	KF576645	100	17	46	87	36	64	0	1	24	13	50	4	175	71	2895	896
OTU_25	11 394	Didymella phacae	NR_135942	99,26	1116	461	996	533	576	266	1753	1387	958	599	161	843	612	477	656
OTU_41	1414	Pithomyces chartarum	KT898697	100	233	23	77	126	40	54	196	214	118	40	25	65	67	51	85
OTU_60	23 305	Penicillium olsonii	JQ781797	100	841	162	12 678	1011	7675	36	227	278	54	82	7	57	101	37	59
OTU_62	14 924	Ochrocladosporium elatum	GU248334	94,53	2973	1189	1763	1194	961	682	1476	1154	652	530	209	607	501	676	357
OTU_63	5247	Botryotinia fuckeliana	KX463512	100	1959	147	1025	211	675	5	161	239	50	77	5	148	226	209	110
OTU_71	1159	Trametes versicolor	KR261581	100	4	23	79	45	47	12	304	164	73	57	17	34	79	131	90
OTU_78	2017	Phaeothecoidea melaleuca	HQ599594	90,32	253	157	249	268	170	78	320	113	78	55	27	102	75	42	30
OTU_86	1715	Nigrospora oryzae	KT898587	100	238	104	93	25	51	20	302	110	273	41	7	56	133	168	94
OTU_96	1026	Dothiora laureolae	KU728503	98,37	275	42	305	97	188	23	6	11	0	16	8	33	7	3	12
OTU_100	8789	Fomitopsis pinicola	AY854083	99,71	0	2	0	0	0	0	1067	1515	118	113	28	4618	36	649	643
OTU_115	2219	Phoma medicaginis	KC492458	97,75	120	65	356	462	180	50	226	274	37	49	16	71	117	119	77
OTU_705	456	Heterobasidion irregulare	KX298233	100	31	19	1	12	0	0	199	63	28	9	13	21	48	3	9
OTU_5022	29	Heterobasidion occidentale	KC492948	98,42	6	2	2	3	0	0	0	10	2	1	1	0	0	2	0
OTU_5273	28	Heterobasidion occidentale	KC492948	98,42	15	3	1	1	0	0	0	6	0	1	0	0	0	0	1
OTU_7272	16	Heterobasidion occidentale	KC492948	98,4	0	1	1	1	0	0	0	10	0	2	0	0	0	0	1

Fig. 1 Phylogenetic relationship among Heterobasidion DNA reads consensus sequence isolated from nursery plants based on Neighbour-joining analysis on the ITS1 partial 5.8s ribosomal sequence data. Bootstrap percentages are indicated at nodes (10,000 replicates), and branch length is proportional to the number of changes indicated. GenBank accession numbers are indicated after species names.