Figures & data
Fig. 1 Agarose gel electrophoresis of PCR products for detection of Bean golden mosaic virus (BGMV). Primers specific for the DNA-A coat protein gene of the virus were used in the PCR analysis and amplified a 550 bp product. The gel was stained with SYBR Gold nucleic acid gel stain. Lane labelling is as follows: 1Kb – molecular weight marker; 1 – amplification from a bean plant of cultivar ‘Carioca’ showing mosaic and wrinkling symptoms; 2 – positive control; 3 – negative control (no DNA included in reaction mixture)
Table 1. Severity of mosaic symptoms at 7, 10, 15, 20, and 25 days after inoculation of different cultivars and lineages of common bean (Phaseolus vulgaris) and Calopogonium mucunoides with Bean golden mosaic virus.
Table 2. Severity of wrinkling symptoms at 7, 10, 15, 20, and 25 days after inoculation of different cultivars and lineages of common bean (Phaseolus vulgaris) and Calopogonium mucunoides with Bean golden mosaic virus.
Fig. 2 Bean cultivar ‘Carioca’, infected with the Bean golden mosaic virus (BGMV) showing symptoms of: (a) Mosaic and (b) Wrinkling
Table 3. Quantification of the Bean golden mosaic virus by RT q-PCR in different cultivars and lineages of common bean (Phaseolus vulgaris) and Calopogonium mucunoides.