Fusarium head blight resistance in F₁ hybrid spring wheat

Figures & data

Table 1. Descriptions of the parents that were used for hybrid production including Fusiarum head blight (FHB) resistance alleles, year of cultivar release, pedigree, and developing institution

Genotype	Fhb1 (3B)	Fhb5 (5A)	Frontana QTL (3A)	Year of Release	Pedigree	Developer
Alsen (PI 615 543)	+	+	+	2000	ND674//ND2710/ND688	NDSU
Faller (PI 648 350)	+	+	-	2009	ND2857/Dapps	NDSU
SY Soren (PI 662 048)	+	-	-	2011	Norpro/Kelby	Syngenta
Glenn (PI 639 273)	-	-	-	2005	ND2831/Parshall/ND706	NDSU
2398 (PI 593 044)	-	-	-	1995	MN-7357/SD-2903	Pioneer

^a(+) indicates cultivar has the resistant allele, (-) indicates the null allele

Table 2. Combined analysis of variance across the four environments (PRO 18, LANG 19, PRO 19, SAB 19) for four Fusarium head blight (FHB) resistance-related traits (FHB Index, Fusarium Damaged Kernels, DON concentration, DISK score)

	Index		FDK		DON		DISK
Sources	df	MS	df	MS	df	MS	df	MS
REP(ENVIRONMENT)	11	169.6	11	737.4**	11	278 555.6**	11	24 335.3**
GENOTYPE	10	7631.5**	10	17 240.1**	10	100 200.7**	10	22 317.8**
ENVIRONMENT	3	14 607.9**	3	52 860.2**	3	1 420 657.7**	3	190 529.6**
GENOTYPE*ENVIRONMENT	30	1110.9**	30	1096.5**	30	44 471.4**	30	4195.0**
ERROR	587	101.7	583	71.3	584	4933.0	583	473.3
R^2		0.77		0.91		0.78		0.82
CV		26.0		22.1		79.1		33.4

Table 3. Least square means and least significant difference (LSD) mean separation for the four response traits (Fusarium head blight (FHB) Index, Fusarium Damaged Kernels, DON concentration, DISK score) of the 11 genotypes included in this study, across all four locations

Name	FHB Index	FDK	DON	DISK
2398	71.2a	66.1b	136.9b	95.1b
WBMayville	66.3b	84.3a	214.6a	121.6a
SY Soren	45.7 c	36.7e	83.8 c	64.9d
2398*SY Soren	40.3d	50.3 c	120.0b	78.8 c
2398*Glenn	39.3d	44.2d	115.7b	75.0 c
2398*Alsen	34.8e	37.3e	75.5 c	60.2d
Glenn	34.6e	25.7 g	76.7 c	56.7d
2398*Faller	34.1e	44.1d	127.7b	77.9 c
Alsen	31.9e	17.2 h	43.0d	44.0e
Faller	31.5e	31.1 f	86.7 c	60.4d
ND2710	22.5 f	11.1i	34.9d	35.5e

Table 4. Contrast analyses of hybrid genotypes with the resistant, susceptible, and mid-parent value for the four Fusarium head blight (FHB) resistance-related traits, by location and combined across locations

Comparisons	Estimates
	LAN-2019	PRO-2018	PRO-2019	SAB-2019	Combined
	Fusarium head Blight (FHB) Index
Alsen (-) Alsen Hybrid	−1.4	−7.3*	−4.0	1.2	−2.9
2398 (-) Alsen Hybrid	20.2**	63.9**	22.1**	39.4**	36.4**
Mid-parent (-) Alsen Hybrid	18.8**	56.6**	18.1*	40.7**	33.5**
Faller (-) Faller Hybrid	−9.0**	−3.6	1.5	0.5	−2.6
2398 (-) Faller Hybrid	23.8**	64.3**	21.5**	38.7**	37.0**
Mid-parent (-) Faller Hybrid	14.9**	60.6**	23**	39.2**	34.4**
Glenn (-) Glenn Hybrid	0.7	−10.3**	−3.1	−6.1	−4.7**
2398 (-) Glenn Hybrid	17.7**	61.8**	17.5**	30.5**	31.8**
Mid-parent (-) Glenn Hybrid	18.4**	51.5**	14.4*	24.4**	27.2**
SY Soren (-) SY Soren Hybrid	3.4	6.2	11.3*	0.9	5.4**
2398 (-) SY Soren Hybrid	17.5**	62.1**	14.2**	30**	30.9**
Mid-parent (-) SY Soren Hybrid	20.9**	68.4**	25.4**	30.9**	36.4**
	Fusarium Damaged Kernels (FDK)
Alsen (-) Alsen Hybrid	−6.1*	−12**	−35.9**	−26.1**	−20**
2398 (-) Alsen Hybrid	52.8**	17.7**	17.5**	27.5**	28.9**
Mid-parent (-) Alsen Hybrid	46.7**	5.7*	−18.3*	1.3	8.8**
Faller (-) Faller Hybrid	−11.7**	−7.4**	−15.7**	−16.9**	−13**
2398 (-) Faller Hybrid	44.7**	17.0**	9.9	16.6**	22.1**
Mid-parent (-) Faller Hybrid	33.0**	9.6**	−5.8	−0.3	9.1**
Glenn (-) Glenn Hybrid	−6.4*	−11.8**	−41.9**	−13.9**	−18.5**
2398 (-) Glenn Hybrid	53.6**	13.5**	1.4	19.4**	22**
Mid-parent (-) Glenn Hybrid	47.2**	1.7	−40.5**	5.5	3.5
SY Soren (-) SY Soren Hybrid	−8.9**	−10.1**	−18.2**	−17.2**	−13.6**
2398 (-) SY Soren Hybrid	38.9**	13.5**	3.0	8.0	15.8**
Mid-parent (-) SY Soren Hybrid	30.0**	3.4	−15.3	−9.2	2.2
	Deoxynivalenol (DON)
Alsen (-) Alsen Hybrid	21.8	−9.7**	−25.1**	−116.9*	−32.5*
2398 (-) Alsen Hybrid	97.1**	−6.8**	−7.2	162.2**	61.3**
Mid-parent (-) Alsen Hybrid	118.9*	−16.5**	−32.3**	45.3	28.8
Faller (-) Faller Hybrid	0.4	−5.9**	−30.8**	−127.7*	−41**
2398 (-) Faller Hybrid	83.9**	−9.7**	−56.8**	19.1	9.1
Mid-parent (-) Faller Hybrid	84.3	−15.6**	−87.6**	−108.7	−31.9
Glenn (-) Glenn Hybrid	−18.6	−10.5**	−28.6**	−98.1*	−38.9**
2398 (-) Glenn Hybrid	48.9	−7.7**	−20**	63.5	21.2
Mid-parent (-) Glenn Hybrid	30.3	−18.1**	−48.6**	−34.6	−17.8
SY Soren (-) SY Soren Hybrid	19.7	−6.5**	−38.2**	−119.8*	−36.2**
2398 (-) SY Soren Hybrid	58.9	−5.3**	−32**	45.8	16.8
Mid-parent (-) SY Soren Hybrid	78.5	−11.8**	−70.2**	−73.9	−19.4
	DISK
Alsen (-) Alsen Hybrid	4.6	−7.7**	−19.2**	−42.5*	−16.2**
2398 (-) Alsen Hybrid	50.1**	16.8**	7.8*	64.9**	34.8**
Mid-parent (-) Alsen Hybrid	54.7**	9.0**	−11.4*	22.3	18.6**
Faller (-) Faller Hybrid	−8.6	−4.7**	−14.2**	−42.7*	−17.6**
2398 (-) Faller Hybrid	44.8**	15.3**	−10.3**	18.9	17.2**
Mid-parent (-) Faller Hybrid	36.1*	10.6**	−24.4**	−23.8	−0.4
Glenn (-) Glenn Hybrid	−6.4	−9.5**	−21.8**	−34.8*	−18.1**
2398 (-) Glenn Hybrid	36.5**	14.9**	−2.0	31.0	20.1**
Mid-parent (-) Glenn Hybrid	30.0*	5.4**	−23.8**	−3.9	1.9
SY Soren (-) SY Soren Hybrid	4.0	−3.8**	−14.6**	−41.0*	−13.9**
2398 (-) SY Soren Hybrid	33.7**	15.3**	−5.8	22.1	16.3**
Mid-parent (-) SY Soren Hybrid	37.7*	11.5**	−20.4**	−18.9	2.4

Fig. 1 Cluster analysis using Ward’s minimum variance method to organize the eleven genotypes into three clusters based on the FHB Index, Fusarium Damaged Kernels, DON concentration, and DISK score, across the four environments

Table 5. Mean and standard deviation of wheat genotypes grouped under the three clusters created using four Fusarium head blight (FHB) resistance-related traits (FHB Index, Fusarium Damaged Kernels (FDK), DON concentration and DISK score)

Cluster	FHB Index	FDK	DON	DISK
1	65.7 ± 4.5	75.2 ± 9.4	175.7 ± 20.5	108.3 ± 10.0
2	31.1 ± 4.1	24.5 ± 4.8	63.4 ± 16.7	35.5 ± 5.5
3	39.9 ± 5.5	36.7 ± 9.1	111.8 ± 38.9	75.2 ± 13.2

Fig. 2 FHB Index, Fusarium Damaged Kernels, DON concentration and DISK score of four F1 hybrids, their moderately resistant parent, the susceptible parent (‘2398’), and mid-parent value, across four environments