Differential reaction of hexaploid and tetraploid wheat to Fusarium graminearum chemotypes in a controlled environment

Figures & data

Table 1. Hexaploid and tetraploid wheat genotypes included in this study and their response to Fusarium head blight (FHB)

Genotype	Species	Reaction^a	Reference
ND2710	Hexaploid	R	Frohberg et al. (2004); McCartney et al. (2004)
‘AC Barrie’	Hexaploid	I	McCaig et al. (1996); McCartney et al. (2004)
‘CDC Teal’	Hexaploid	S	Hughes and Hucl (1993)
BGRC3487	Tetraploid	R	Ruan et al. (2012); Brar (2019)
DT696	Tetraploid	I	Clarke et al. (2010)
DT735	Tetraploid	I	Clarke et al. (2010); Ruan et al. (2012); Brar (2019)
‘AC Morse’	Tetraploid	S	Clarke et al. (2010)

^aReaction type to FHB: R = resistant, I = intermediate, MS = moderately susceptible, and S = susceptible.

Table 2. Mixed analysis for disease scale (DS) and percentage of infected spikelets (IS) at the endpoint of Fusarium head blight disease development (day 21)

		IS (%)	DS (0–5)
Fixed effects	df	F-value
Genotype	6	351.9***	139.7***
Chemotype	2	44.2***	26.3***
Genotype*Chemotype	12	6.9***	4.3***
Random effects		Variance
Replication (Experiment)		0	0
Experiment		0	0
Experiment*Chemotype		0	0
Experiment*Genotype		7.33*	0.01*
Experiment*Genotype*Chemotype		0.03ns	0.01ns

Note. *^, **^, *** Significant at P < 0.05, P < 0.01 and P < 0.001, respectively; ns, not significant.

Table 3. Least-squared means of percentage of infected spikelets (%) of hexaploid and tetraploid wheat genotypes in response to 3-acetyl-deoxyinvalenol (ADON) and 15-ADON chemotypes of Fusarium graminearum, and a mixture of the two chemotypes across isolates

		Hexaploid			Tetraploid
Experiment	Chemotype	ND2710	AC Barrie	CDC Teal	BGRC3487	DT696	DT735	AC Morse
First	3-ADON	7.5	64.0	89.1	6.2	15.9	26.6	91.0
	15-ADON	9.5	53.9	86.7	10.2	28.0	31.3	95.8
	Mixture	5.6	41.0	85.0	4.8	16.3	16.6	82.6
Second	3-ADON	12.8	60.2	92.5	5.6	21.4	29.4	84.7
	15-ADON	8.5	49.7	89.1	13.2	35.1	37.2	98.4
	Mixture	5.7	32.8	83.1	4.8	13.4	25.5	92.6
Third	3-ADON	13.1	53.8	96.2	5.5	31.0	18.0	91.4
	15-ADON	9.0	46.3	92.1	8.5	42.4	33.9	98.0
	Mixture	6.0	42.1	83.1	7.7	25.0	21.1	92.5
Mean of Experiments		8.6	49.3	88.5	7.4	25.3	26.6	91.9
LSD^a (0.05) within an experiment					6.7 (first); 6.9 (second); 7.1 (third)
LSD (0.05) over experiments					4.7

^aLSD, least significant difference at P < 0.05.

Table 4. Effects averaged over experiments for two 3-acetyl-deoxyinvalenol (ADON) isolates, two 15-ADON isolates and two chemotype mixtures of Fusarium graminearum on percentage of infected spikelets (%) for disease response in hexaploid and tetraploid wheat genotypes

	3-ADON			15-ADON			Mixture
Genotype	M6-04-4	M9-04-6	Mean	M1-04-1	M8-04-3	Mean	M6M1	M9M8	Mean
Hexaploid
ND2710	10.8	11.5	11.2	8.2	9.8	9.0	5.8	5.8	5.8
AC Barrie	62.3	56.3	59.3	51.6	48.3	50.0	35.3	42.0	38.7
CDC Teal	92.9	92.3	92.6	89.3	89.3	89.3	81.0	86.4	83.7
Tetraploid
BGRC3487	5.1	6.4	5.7	11.0	10.2	10.6	5.2	6.3	5.8
DT696	19.9	25.6	22.8	37.2	33.1	35.1	20.4	16.0	18.2
DT735	23.6	25.6	24.6	37.6	30.7	34.1	20.6	21.5	21.0
AC Morse	90.0	88.1	89.0	97.5	97.3	97.4	88.7	89.8	89.2
LSD^a (0.05) within a genotype					6.7 (isolate); 3.1 (mean)
LSD (0.05) for other comparisons					7.1 (isolate); 5.8 (mean)

Note. M6M1: M6-04-4 and M1-04-1; M9M8: M9-04-6 and M8-04-3.

^aLSD, least significant difference at P < 0.05.

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