Evidence for a genetically distinct population of Exobasidium sp. causing atypical leaf blight symptoms on lowbush blueberry (Vaccinium angustifolium Ait.) in Newfoundland and Labrador, Canada

Figures & data

Table 1. List of isolates included in this study

Species	Host	Collection location^a	Isolate code	Accession (ITS/LSU)^b	Reference (ITS/LSU)
Exobasidium sp.	V. angustifolium	Flatrock, NL	2017.07.14_SC_02	MW051429/NA	This study
		Witless Bay, NL	2017.07.23_TT_03	MW051430/MW462223	This study
		Avondale, NL	2017.06.30_AV_01	MW051431/NA	This study
		Flatrock, NL	2017.07.14_SC_05b	MW051432/NA	This study
		Makinsons, NL	2017.07.10_RL_02	MW465842/MW462224	This study
E. maculosum	V. angustifolium	Kings Co., NS	E86-1	KR262419.1/KF134420	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. angustifolium	Lunenburg Co., NS	E92-50	KR262418.1/KF134425	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. angustifolium	Waldo Co, ME, USA	ME1-13	KR262417.1/KF134412	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. angustifolium	Kings Co., NS	E88-9	KR262416.1/KF134421	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. corymbosum cv. Duke (HB)	Sampson County, NC, USA	E12NCLC1-44	KKFR262353/KF134414	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. corymbosum cv. Duke (HB)	Sampson County, NC, USA	E12NCLC1-45	KR262387/KF134416	Stewart et al. (2015)/Brewer et al. (2014)
E. maculosum	V. corymbosum hybrid (S Star)	Bacon Co., GA, USA	E6-2	KR262342/NA	Stewart et al. (2015)
E. maculosum	V. corymbosum hybrid (S Star)	Bacon Co., GA, USA	FS2-4	KR262385/NA	Stewart et al. (2015)
E. maculosum	Vaccinium virgatum	Bacon Co., GA, USA	ATCC:MYA-4957/E12A1-1	NA/NG_059219.1	Brewer et al. (2014)
E .rostrupii	V. oxycoccos	Poland	TUB 019165	FJ896132.1/FJ896137	Piątek et al. (2012)
E. rostrupii	V. macrocarpon	Burlington Co., NJ, USA	CNJ2-3	KR262425.1/NA	Stewart et al. (2015)
E. rostrupii	V. macrocarpon	Burlington Co., NJ, USA	E12CNJ1-1	NA/KF134408	Brewer et al. (2014)
E. rostrupii	V. macrocarpon	Kings Co., NS	E80-1	NA/KF134417	Brewer et al. (2014)
E. rostrupii	Oxycoccus palustris	Poland	TUB 019165	NA/FJ896137.1	Piątek et al. (2012)
E. rostrupii	Vaccinium oxycoccos	Bayern, Germany	PB 3275	NA/DQ025494.1	Unpublished
E. rostrupii	Vaccinium oxycoccos	Unknown	RB 949	NA/AF009857.1	Begerow et al. (1997)
E. pachysporum	V. uliginosum	Japan	MAFF 238621	AB180352.1/AB177573	Unpublished
E. uvae-ursi	Arctostaphylos uva-ursi	Unknown	GLM-F105774	KY424482.1/NA	Unpublished
E. arescens	V. myrtillus	Slovenia	TUB 015031	FJ896135.1/FJ896136	Piątek et al. (2012)
E. darwinii	V. reticulatum	Maui, HI, USA	TUB 019166	NA/FJ896134.1	Piątek et al. (2012)
E. ferrugineae	Lyonia ferruginea	Florida, USA	BPI:882571	NR_120076.1/JQ611711.1	Kennedy et al. (2012)
E. canadense	Rhododendron mariesii	Unknown	CGMCC 5.1647	EU692771.1/EU692791.1	Li and Guo (2009)
Uncultured fungus	Cheirodendron trigynum	Hawaii, USA	CheiRtLf1719	NA/JX241787.1	Ort et al. (2012)
Meira geulakonigii	Phyllocoptruta oleivora (Ashmead) (Eriophyidae on Citrus grandis)	Dan, Israel	CBS:110052	NR_073343.1/NG_042394.1	Boekhout et al. (2003)

^aCountry of origin is Canada unless otherwise stated.

^bThe abbreviation NA means that an ITS or LSU sequence was unavailable for the strain in question. Type strains are designated by bolded accession numbers.

Fig. 1 (Colour online) Atypical Exobasidium-leaf-spot-like disease symptoms on Vaccinium angustifolium caused by Exobasidium sp. Symptoms on wild plants in Holyrood, NL, July 23, 2018 (a); close-up of atypical symptoms on detached leaf of V. angustifolium showing large, raised lesion (b); typical Exobasidium leaf spot symptoms on detached leaf of V. angustifolium (c); two-month old colony of Exobasidium sp., isolated from atypical leaf spot symptoms, on PDA+AB after 2 months of growth at 23 °C in the dark (d)

Fig. 2 (Colour online) Neighbour-joining trees depicting the relationship between sequences from Exobasidium spp. isolates originally collected from Vaccinium spp. and related host species (Table 1). Bootstrap values are expressed as percentages on the nodes of each branch. Colours indicate the host from which the isolate was originally obtained: black text represents samples collected from V. angustifolium; blue text represents isolates from V. virgatum or V. corymbosum; red text indicates isolates from other Vaccinium spp.; green text indicates isolates from other Ericaceae; and brown text indicates a host origin outside the Ericaceae. Isolates in bold were collected in this study, and an asterisk indicates type strains. Sequences from either (a) ITS or (b) LSU were used to construct each tree

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