Isolation and identification of Colletotrichum truncatum causing anthracnose disease of the Chinese medicinal herb Cynanchum paniculatum

Figures & data

Fig. 1 Symptoms of anthracnose disease in C. paniculatum caused by C. truncatum. a-d. healthy herbs and pods. e. pod lesions. f. leaf spots. g. stem blight. h, inoculated pod. i, inoculated leaf.

Table 1. Primers used for PCR amplification and DNA sequencing.

Gene	Primer	Sequence (5’-3’)	Reference
ITS	TIS1 TIS4	TCCGTAGGTGAACCTGCGG TCCTCCGCTTATTGATATGC	White et al. (1990)
actin	ACT-512 F ACT-783 R	ATGTGCAAGGCCGGTTTCGC TACGAGTCCTTCTGGCCCAT	Carbone and Kohn (1999)
β-tubulin	Bt2a Bt2b	GGTAACCAAATCGGTGCTGCTTTC ACCCTCAGTGTAGTGACCCTTGGC	Glass and Donaldson (1995)
histone	CYLH3F CYLH3R	AGGTCCACTGGTGGCAAG AGCGATGTCCTTGGACTG	Crous et al. (2004)

Table 2. Pathogenicity test of Colletotrichum truncatum isolate XTJ03.

Host	Cultivated species or location	Host tissue	Symptom severity	Control
Cynanchum paniculatum	Yimeng, Shandong province	Leaf	Moderate	ND
		Bean pod	Heavy	ND
		Stem	Heavy	ND
Glycine max	Zhonghuang-57	Pod	Heavy	ND
Phaseolus vulgaris	Duojia-2	Pod	Moderate	ND
Armeniaca vulgaris	Hongyu	Fruit	Heavy	ND
Solanum lycopersicum	Maofen-808	Fruit	Heavy	ND
Capsicum annuum	Shenglong-158	Fruit	Moderate	ND
Amygdalus persica	Zhonghua	Fruit	Moderate	ND
Fragaria ananassa	Xinxing	Fruit	Moderate	ND
Mangifera indica	Tianyang	Fruit	Moderate	ND
Malus domestica	Red Fuji	Fruit	ND	ND

ND means no detected

Fig. 2 Microscopic and colonial morphology of C. truncatum isolate XTJ03. a,b. acervulus. c. appressoria. d,e. colonies on PDA above and below. f. conidiophores.

Fig. 3 Phylogenetic tree based on the sequences of ITS, actin, β-tubulin and histone genes of XTJ03 and related species. Gene sequences for the related species were obtained from Genbank. Colletotrichum coccodes was used as an outgroup.

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