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Figures & data
Table 1. Oligonulceotide primers used in the study.
Table 2. Infectivity of PeLCV and PeLCV bearing mutations of the virion-sense (V2 and CP) and complementary-sense (C2 and C4) genes in Nicotiana benthamiana in the presence and absence of TbLCB.
Fig. 1 Symptoms exhibited by Nicotiana benthamiana plants after inoculation with Pedilanthus leaf curl virus (PeLCV) and PeLCV harbouring mutations of the V2 (PeΔV2) and CP (PeΔCP) genes, with or without tobacco leaf curl betasatellite (Tβ). The N. benthamiana plants shown were either not inoculated (healthy; A) or inoculated with PeLCV (B), Pe and Tβ (C), PeΔV2 (D), PeΔV2 and Tβ (E,F), PeΔCP (G), PeΔCP and Tβ (H,I). Photographs were taken at 25 dpi.
Fig. 2 Estimation of the PeLCV titre (ng µg−1 of total DNA) in the newly emerging leaves of the inoculated Nicotiana benthamiana plants. Plants were inoculated with PeLCV and its gene mutants (V2, CP, TrAP and C4) along with a non-inoculated N. benthamiana plant (negative control; not shown). Each bar graph is a technical-replicate of three mean values (shown in dotted green line), while the standard deviation is represented by the bar and the median by the line. Bars with different letters are statistically different from each other (p ≤ 0.05).
Fig. 3 Symptoms exhibited by Nicotiana benthamiana plants after inoculation with Pedilanthus leaf curl virus (PeLCV) and PeLCV harbouring mutations of the C2 and C4 genes, with and without tobacco leaf curl betasatellite (Tβ). The N. benthamiana plants shown were either not inoculated (healthy; A) or inoculated with PeLCV (B), PeLCV and Tβ (C), PeΔC2 (D), PeΔC2 and Tβ (E,F), PeΔC4 (G), PeΔC4 and Tβ (H,I). Photographs were taken at 25 dpi.
