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Journal of the American College of Nutrition Volume 23, 2004 - Issue 5
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SUPPLEMENT: TENTH INTERNATIONAL MAGNESIUM SYMPOSIUM

The Determination of Brain Magnesium and Zinc Levels by a Dual-Probe Microdialysis and Graphite Furnace Atomic Absorption Spectrometry

Dar-Yu Yang Department of Emergency (D.Y.Y.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, MD, MSC,
Jen-Bin Lee Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Taichung Veterans General Hospital, Department of Applied Chemistry, Providence University (J.B.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, MS,
Ming-Cheng Lin Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Graduate Institute of Medicine, (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, MS,
Yeou-Lih Huang School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, PhD,
Hong-Wen Liu Department of Emergency (D.Y.Y.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Taichung Veterans General Hospital, Department of Applied Chemistry, Providence University (J.B.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Graduate Institute of Medicine, (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, MD,
Yea-Jiuan Liang Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN
, MS &
Fu-Chou Cheng Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWANCorrespondence[email protected]
, PhD show all
Pages 552S-555S | Received 05 Aug 2004, Published online: 18 Jun 2013
 
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Abstract

Objective: The aim of this study was to develop a microdialysis-graphite furnace atomic absorption spectroscopy (MD-GFAAS) for monitoring dynamic changes of extracellular magnesium (Mg) and zinc (Zn) in the cortex of gerbils subjected to focal cerebral ischemia, that had been produced in anesthetized gerbils by occlusion of the right middle cerebral artery.

Methods: Two microdialysis probes were inserted into both sides of the cortex to simultaneously collect dialysates during cerebral ischemia. Dynamic changes in these analytes, on ipsilateral and contralateral sides of the brain, were assayed by MD-GFAAS. Optimal conditions and analytical precision of GFAAS were studied in the present assay.

Results: The present study demonstrated significant decreases in Mg (65% of baseline) and zinc (74% of baseline) maintained their levels within 3 h on the ipsilateral side of cortex during cerebral ischemia. Slight changes of Mg and Zn on the contralateral sides were also observed.

Conclusion: The derangement of extracellular Mg and Zn could be important in the progression of cell injury and may be associated with cerebral ischemia insult.

This study was supported by grants from the Taichung Veterans General Hospital (TCVGH-927207C & 927316D) Taiwan, Republic of China.

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