Synthesis of S-sialyl polymers as efficient polyvalent influenza inhibitors and capturers

GRAPHICAL ABSTRACT

ABSTRACT

Glycopolymers with different sugar densities were prepared by reacting monomeric S-sialoside with polymers carrying maleic anhydride moieties. The glycoconjugates as mucin mimics competitively inhibit viral infection through multivalent interactions with hemagluttinin and neuraminidase as sialic acid receptor, which were evaluated using hemagglutination aggregation, neuraminidase inhibition assays and isothermal titration calorimetry. These glycopolymers can also be potentially used as capture molecules for a wide variety of viral strains. We anticipate that these S-sialyl polymers with strong bindings to influenza virus can not only be used as antiviral agents but also tools for the study of sialic acid-influenza virus recognition, interaction and capture.

KEYWORDS:

• Multivalent effect
• Glycopolymer
• Isothermal titration calorimetry
• Hemagluttinin inhibitor
• Neuraminidase inhibitor

Acknowledgments

This work was financially supported by the Natural Science Foundation of China (21402140, 21502139), Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education and Tianjin Key Lab of Industrial Microbiology (2015IM107) and Youth Innovation Research Foundation of Tianjin University of Science and Technology (2016LG08). The authors are thankful to the Research Centre of Modern Analytical Technology, Tianjin University of Science and Technology for NMR measurements. The authors also appreciate National Institute for Viral Disease Control and Prevention, China CDC for the viral strains.

Additional information

Funding

The International Science & Technology Cooperation Program of China(2013DFA31160), Natural Science Foundation of China(21402140, 21502139), Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education and Tianjin Key Lab of Industrial Microbiology(2015IM107), Youth Innovation Research Foundation of Tianjin University of Science and Technology(2016LG08)