Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution

Figures & data

Figure 1. Stability of viral nucleic acids. Viruses remain stable in ultra-low freezers (left) and at room temperature (right), except for SARS-CoV-2, which rapidly declines over time at room temperature, becoming undetectable after 2 months.

Table 1. Contingency table for external quality assurance panels detected by each formulation and summary statistics.

		VPSS2
		Positive	Negative	Total
VPSS	Positive	266	2	268
	Negative	2	54	56
	Total	268	56	324
Summary statistics		Percent	Low limit	High limit
Positive agreement (PPA)		99.3%	97.3%	99.8%
Negative agreement (PNA)		96.4%	87.9%	99.0%
Overall agreement (POA)		98.8%	96.9%	99.5%

PPA = [a/(a+c)]*100; PNA = [d/b+d))]*100; POA = [(a+d)/n]*100.

VPSS2 false negatives: lab code GB240, sample VSERSCV2_21-20 and 21.

VPSS false negative: lab code GB003, sample VSERSCV2_21-09 and lab code GB225, sample VSERSCV2_21-05.